BLMH/Bleomycin Hydrolase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88640

Novus Biologicals, part of Bio-Techne
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NBP1-88640
NBP1-88640-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BLMH/Bleomycin Hydrolase Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RKEPEDGRLVQFLLMNPANDGGQWDMLVNIVEKYGVIPKKCFPESYTTEATRRMNDILNHKMREFCIRLRNLVHSGATKGEISATQDVMMEEIFRVVCICLGNP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BLMH/Bleomycin Hydrolase Antibody

Western Blot: BLMH/Bleomycin Hydrolase Antibody [NBP1-88640]

Western Blot: BLMH/Bleomycin Hydrolase Antibody [NBP1-88640]

Western Blot: BLMH/Bleomycin Hydrolase Antibody [NBP1-88640] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: BLMH/Bleomycin Hydrolase Antibody [NBP1-88640]

Immunohistochemistry-Paraffin: BLMH/Bleomycin Hydrolase Antibody [NBP1-88640]

Immunohistochemistry-Paraffin: BLMH/Bleomycin Hydrolase Antibody [NBP1-88640] - Staining of human testis.
Western Blot: BLMH/Bleomycin Hydrolase Antibody [NBP1-88640]

Western Blot: BLMH/Bleomycin Hydrolase Antibody [NBP1-88640]

Western Blot: BLMH/Bleomycin Hydrolase Antibody [NBP1-88640] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
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Applications for BLMH/Bleomycin Hydrolase Antibody

Application
Recommended Usage

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BLMH/Bleomycin Hydrolase

Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq]

Alternate Names

BMH

Gene Symbol

BLMH

Additional BLMH/Bleomycin Hydrolase Products

Product Documents for BLMH/Bleomycin Hydrolase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BLMH/Bleomycin Hydrolase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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