BLNK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-804

Novus Biologicals, part of Bio-Techne
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NB100-804
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all BLNK Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KDSTRLKYAVKVS corresponding to C-Terminus according to NP_037446.1, NP_001107566.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_037446.1; NP_001107566.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for BLNK Antibody

Western Blot: BLNK Antibody [NB100-804]

Western Blot: BLNK Antibody [NB100-804]

Western Blot: BLNK Antibody [NB100-804] - Staining of Daudi cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: BLNK Antibody [NB100-804]

Immunocytochemistry/ Immunofluorescence: BLNK Antibody [NB100-804]

Immunocytochemistry/Immunofluorescence: BLNK Antibody [NB100-804] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing some membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: BLNK Antibody [NB100-804]

Flow Cytometry: BLNK Antibody [NB100-804]

Flow Cytometry: BLNK Antibody [NB100-804] - Flow cytometric analysis of paraformaldehyde fixed Daudi cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for BLNK Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/mL
Application Notes
WB: Approx. 70 kDa band observed in Daudi lysates (calculated MW of 50.5 kDa band according to NP_037446.1). The observed molecular weight corresponds to how SLP-65 is expected to run. However, when transiently expressed in HEK293, a 50 kDa band is observed.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BLNK

BLNK (B cell linker protein) is an adaptor molecule that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). This activation leads to an increase in the tyrosine phosphorylation of a number of proteins due to activation of nonreceptor protein tyrosine kinases (PTK) including Syk, which is responsible for the tyrosine phosphorylation of BLNK. BLNK is cytosolic protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human BLNK). Mapping of the tyrosine residues in BLNK reveal three tyrosines that bind PLC-gamma2, and two tyrosines that bind the Tec family kinase Btk, the Vav nucleotide exchange factor and the adaptor Nck.

Long Name

B-cell Linker Protein

Alternate Names

BASH, Ly57, SLP65

Entrez Gene IDs

29760 (Human); 17060 (Mouse)

Gene Symbol

BLNK

UniProt

NP_037446. (Human)

Additional BLNK Products

Product Documents for BLNK Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BLNK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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