BMAL1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-129

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB100-129SS
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Chromatin Immunoprecipitation (ChIP), Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all BMAL1 Antibodies »

Product Specifications

Immunogen

Recombinant protein corresponding to residues 294-624 of human BMAL1. [UniProt# O00327]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BMAL1 Antibody

Western Blot: BMAL1 Antibody [NB100-129]

Western Blot: BMAL1 Antibody [NB100-129]

Western Blot: BMAL1 Antibody [NB100-129] - Detection of BMAL in 3T3/L1 lysates.
Simple Western: BMAL1 Antibody [NB100-129]

Simple Western: BMAL1 Antibody [NB100-129]

Simple Western: BMAL1 Antibody [NB100-129] - Image shows a specific band for BMAL1 in 0.5 mg/mL of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for BMAL1 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Simple Western

1:100

Western Blot

1:100 - 1:200
Application Notes
This BMAL1 antibody is useful for Chromatin Immunoprecipitation and Western blot, where it recognizes a band at approx. 73 kDa representing BMAL1. Results were negative for ICC/IF in Hela and MCF7 cells.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BMAL1

BMAL1 (brain and muscle ARNT-like 1; ARNTL) is an essential core component in circadian clock machinery which is regulatory mechanism for circadian rhythms. Circadian clock genes include three period proteins (PER1, PER2 and PER3), two cryptochromes (CRY1 and CRY2), CLOCK, NPAS2 and BMAL proteins, wherein BMAL1 has a potential to heterodimerize with CLOCK or NPAS2 genes; and this neocomplex drives transcription from E-box elements (5'-CACGTG-3') found in circadian-responsive genes's promoters. PER/CRY proteins negatively regulate CLOCK/BMAL1 dimer-mediated transcription, thereby forming the feedback loop that regulates the timing of clock gene transcription. BMAL1 also associates with GNB2L1/RACK1 and PRKCA in a nuclear complex, whertein GNB2L1 and PRKCA are recruited to the complex in a circadian manner. BMAL1 undergoes acetylation (Lys-538), phosphorylation and sumoylation (Lys-259) upon dimerization with CLOCK and acetylation facilitates CRY1-mediated repression. CLOCK-BMAL1 double mutations within PAS domains leads to syngernistic desensitization to high levels of CRY on repression of CLOCK-BMAL1 transcriptional activity of PER1 and, disrupt circadian rhythmicity. Clock genes functions primarily as tumor suppressors and their abbarant expression is observed in malignant pleural mesothelioma, colorectal and breast cancer.

Long Name

Brain and Muscle ARNT-Like 1

Alternate Names

ARNTL, BHLHE5, BMAL1c, JAP3, MOP3, PASD3, TIC

Entrez Gene IDs

406 (Human)

Gene Symbol

ARNTL

UniProt

O00327

Additional BMAL1 Products

Product Documents for BMAL1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BMAL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.