BMI-1 Antibody (1F2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26477
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1F2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human BMI-1 [UniProt P35226]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BMI-1 Antibody (1F2)
Immunohistochemistry: BMI-1 Antibody (1F2) [NBP3-26477] -
Immunohistochemistry: BMI-1 Antibody (1F2) [NBP3-26477] - Image of BMI-1 Antibody (1F2) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: BMI-1 Antibody (1F2) [NBP3-26477] -
Immunohistochemistry: BMI-1 Antibody (1F2) [NBP3-26477] - Image of BMI-1 Antibody (1F2) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: BMI-1 Antibody (1F2) [NBP3-26477] -
Western Blot: BMI-1 Antibody (1F2) [NBP3-26477] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, THP-1 whole cell lysate, U87 whole cell lysate, U251 whole cell lysate.All lanes: BMI-1 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 37 kDa
Observed band size: 45 kDa
Applications for BMI-1 Antibody (1F2)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: BMI-1
Aberrant expression of Bmi-1 has been found in several types of cancer in the bladder, skin, blood, prostate, breast, ovaries, andcolon. Its overexpression is especially pronounced in mantle cell lymphomas. Thereforem Bmi1 antibodies will be useful tools for many cancer development studies.
Long Name
B Lymphoma Mo-MLV Insertion Region 1
Alternate Names
BMI1, PCGF4, RNF51
Gene Symbol
BMI1
Additional BMI-1 Products
Product Documents for BMI-1 Antibody (1F2)
Product Specific Notices for BMI-1 Antibody (1F2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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