BMI-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-40823

Novus Biologicals, part of Bio-Techne
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NB110-40823
NB110-40823SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (92%), Chicken (92%), Feline (92%), Primate (92%), Rabbit (92%), Rat (92%), Zebrafish (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all BMI-1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region (within residues 1-100) of human Bmi1. [Swiss-Prot# P35226]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: primate, feline, chicken, bovine, rabbit, rat and Zebrafish protein (all 92%).

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BMI-1 Antibody

Western Blot: BMI-1 Antibody [NB110-40823]

Western Blot: BMI-1 Antibody [NB110-40823]

Western Blot: Bmi1 Antibody [NB110-40823] - Detection of Bmi1 protein in skeletal muscle
Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NB110-40823]

Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NB110-40823]

Immunocytochemistry/Immunofluorescence: Bmi1 Antibody [NB110-40823] - BMI1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Applications for BMI-1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

reported in scientific literature (PMID 33798831)

Western Blot

2 ug/ml
Application Notes
In Western blot a band is seen ~37 kDa along with some less intense non-specific bands that do not interfere with the Bmi1 band. In immunocytochemistry/immunofluorescence nuclear foci were detected in HeLa cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Glycine and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BMI-1

The Bmi1 oncogene has been shown to induce telomerase activity and immortalize human mammary epithelial cells. Bmi1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. Bmi1 is also important in maintaining stem cells' ability to self renew. It has also been termed an oncogene with aberrant expression in various carcinomas. It has been shown to be a negative regulator of p19Arf, Ink4a (p14Arf) and tp53 known tumor repressors. Ablation of Bmi1 is required for short term survival of cancer cells.

Long Name

B Lymphoma Mo-MLV Insertion Region 1

Alternate Names

BMI1, PCGF4, RNF51

Gene Symbol

BMI1

Additional BMI-1 Products

Product Documents for BMI-1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BMI-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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