BMI-1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24693
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Primate
Predicted:
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
A portion of amino acids 270-320 of human BMI1 was used as the immunogen.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BMI-1 Antibody
![Western Blot: BMI-1 Antibody [NBP2-24693]](https://resources.bio-techne.com/images/products/BMI-1-Antibody-Western-Blot-NBP2-24693-img0006.jpg "Western Blot: BMI-1 Antibody [NBP2-24693]")
Western Blot: BMI-1 Antibody [NBP2-24693]
Western Blot: BMI-1 Antibody [NBP2-24693] - Analysis of BMI1 in skeletal muscle lysate. Lane 1 shows pre-immune sera. Lanes 2, 3 and 4 show this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP2-24693]](https://resources.bio-techne.com/images/products/BMI-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24693-img0004.jpg "Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP2-24693]")
Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP2-24693]
Immunocytochemistry/Immunofluorescence: BMI-1 Antibody [NBP2-24693] - Immunofluorescence of BMI1 in human oral squamous cell carcinoma (UPCI:SCC029B) cells. These cells are part of a large collection of HNSCC (head and neck SCC) lines established by the Gollin lab (White et al., 2007). The BMI1 antibody was used at 5 ug/ml. Staining is primarily nuclear, some cytoplasmic staining is also observed. Data courtesy of Hatem Kaseb, MD and Susanne M. Gollin, PhD.![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP2-24693]](https://resources.bio-techne.com/images/products/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP2-24693-img0005.jpg "Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP2-24693]")
Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP2-24693]
Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP2-24693] - Analysis of BMI1 in formalin-fixed, paraffin-embedded human cervix tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.Applications for BMI-1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
2.0-3.0 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BMI-1
Aberrant expression of Bmi-1 has been found in several types of cancer in the bladder, skin, blood, prostate, breast, ovaries, andcolon. Its overexpression is especially pronounced in mantle cell lymphomas. Thereforem Bmi1 antibodies will be useful tools for many cancer development studies.
Long Name
B Lymphoma Mo-MLV Insertion Region 1
Alternate Names
BMI1, PCGF4, RNF51
Gene Symbol
BMI1
UniProt
Additional BMI-1 Products
Product Documents for BMI-1 Antibody
Product Specific Notices for BMI-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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