BNIP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-68161

Novus Biologicals, part of Bio-Techne
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NB100-68161
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all BNIP1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DLLRQRKTTKESLAQ corresponding to internal region according to NP_053581.1, NP_053582.1, NP_053583.1.

Epitope

DLLRQRKTTKESLAQ

Specificity

All reported variants represent isoform 1 (NP_001196.1; NP_053581.1; NP_053582.1; NP_053583.1)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for BNIP1 Antibody

Western Blot: BNIP1 Antibody [NB100-68161]

Western Blot: BNIP1 Antibody [NB100-68161]

Western Blot: BNIP1 Antibody [NB100-68161] - (0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for BNIP1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

0.5 - 1.5 ug/ml
Application Notes
WB: Approx. 28 kDa band observed in human kidney lysates (calculated MW of 26.1 kDa band according to NP_001196.1; NP_053581.1; NP_053582.1; NP_053583.1). An additional band of unknown identity was also consistently observed at 48 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BNIP1

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. Alternative splicing of this gene results in four protein products with identical N- and C-termini.

Alternate Names

BCL2/adenovirus E1B 19 kDa protein-interacting protein 1, BCL2/adenovirus E1B 19kDa interacting protein 1, BCL2/adenovirus E1B 19kD-interacting protein 1, Nip1, SEC20, SEC20L, Transformation-related gene 8 protein, TRG-8, vesicle transport protein SEC20

Entrez Gene IDs

662 (Human); 224630 (Mouse); 140932 (Rat)

Gene Symbol

BNIP1

UniProt

NP_001196.1 (Human)

Additional BNIP1 Products

Product Documents for BNIP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BNIP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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