BNP Antibody (JE30-94)

Western Blot: BNP Antibody (JE30-94) [NBP3-32088]

Western Blot: BNP Antibody (JE30-94) [NBP3-32088] - Western blot analysis of BNP on different lysates with Rabbit anti-BNP antibody (NBP3-32088) at 1/1,000 dilution.

Lane 1: SW480 cell lysate (20 ug/Lane)
Lane 2: U-87 MG cell lysate (20 ug/Lane)
Lane 3: U-2 OS cell lysate (20 ug/Lane)
Lane 4: Mouse brain tissue lysate (30 ug/Lane)
Lane 5: Rat brain tissue lysate (30 ug/Lane)

Predicted band size: 15 kDa
Observed band size: 37 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32088) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: BNP Antibody (JE30-94) [NBP3-32088]

Immunohistochemistry: BNP Antibody (JE30-94) [NBP3-32088] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-BNP antibody (NBP3-32088) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32088) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.