BNP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32038

Novus Biologicals, part of Bio-Techne
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NBP1-32038
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BNP Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human BNP. The exact sequence is proprietary.

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BNP Antibody

Immunocytochemistry/ Immunofluorescence: BNP Antibody [NBP1-32038]

Immunocytochemistry/ Immunofluorescence: BNP Antibody [NBP1-32038]

Immunocytochemistry/Immunofluorescence: proBNP Antibody [NBP1-32038] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: BNP protein stained by BNP antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.
BNP Antibody

Western Blot: BNP Antibody [NBP1-32038] -

Western Blot: BNP Antibody [NBP1-32038] - BNP antibody detects BNP protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with BNP antibody (NBP1-32038) diluted by 1:1000.

Applications for BNP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BNP

Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C-type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides, see fig. 18). BNP is a 32 amino acid peptide with disulfide bond between the cystein residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity

Long Name

Brain Natriuretic Peptide

Alternate Names

BNF, NPPB

Gene Symbol

NPPB

Additional BNP Products

Product Documents for BNP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BNP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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