BOB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1122

Novus Biologicals, part of Bio-Techne
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NB100-1122
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Bovine (100%), Canine (100%), Human (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all BOB1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DAYALNHTLSVEGF corresponding to C-Terminus according to NP_006226.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for BOB1 Antibody

Western Blot: BOB1 Antibody [NB100-1122]

Western Blot: BOB1 Antibody [NB100-1122]

Western Blot: BOB1 Antibody [NB100-1122] - Analysis of BOB1 in Mouse Spleen lysate at 2 ug/ ml.
Western Blot: BOB1 Antibody [NB100-1122]

Western Blot: BOB1 Antibody [NB100-1122]

Western Blot: BOB1 Antibody [NB100-1122] - Staining (2ug/ml) of Mouse Spleen lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for BOB1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 30 kDa band observed in mouse spleen lysate (predicted MW of 28 kDa band according to NP_006226).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BOB1

A novel 35kDa protein designated Bob1 (B cell Oct binding protein 1), alternativelt called OBF1, specifically interacts with Oct1 and Oct2, enhancing their transcriptional efficacy. Bob1 is expressed at highest levels in spleen and peripheral blood leukocytes and represents an Oct cofactor capable of conferring cell specific activation of Oct1 and Oct2. Although having no intrinsic capacity for DNA binding, Bob1 associates tightly with the octomer motif in the presence of Oct1 and Oct2.

Alternate Names

B-cell-specific coactivator OBF-1, BOB-1, OBF-1, OBF1BOB1, OCAB, OCA-B, OCT-binding factor 1, POU class 2 associating factor 1, POU domain class 2, associating factor 1, POU domain class 2-associating factor 1, POU domain, class 2, associating factor 1

Entrez Gene IDs

5450 (Human); 18985 (Mouse)

Gene Symbol

POU2AF1

UniProt

NP_006226. (Human)

Additional BOB1 Products

Product Documents for BOB1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BOB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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