Boll Antibody (3E10)

Novus Biologicals, part of Bio-Techne | Catalog # H00066037-M03

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3E10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Boll Antibodies »

Product Specifications

Immunogen

BOLL (NP_149019, 185 a.a. ~ 283 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ATTQYLPGQWQWSVPQPSASSAPFLYLQPSEVIYQPVEIAQDGGCVPPPLSLMETSVPEPYSDHGVQATYHQVYAPSAITMPAPVMQPEPIKTVWSIHY

Reactivity Notes

Other species not tested. Use in Mouse reported in scientific literature (PMID:31963729).

Specificity

BOLL - bol, boule-like (Drosophila)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Boll Antibody (3E10)

Immunohistochemistry-Paraffin: Boll Antibody (3E10) [H00066037-M03]

Immunohistochemistry-Paraffin: Boll Antibody (3E10) [H00066037-M03]

Immunohistochemistry-Paraffin: Boll Antibody (3E10) [H00066037-M03] - Analysis of monoclonal antibody to BOLL on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml
ELISA: Boll Antibody (3E10) [H00066037-M03]

ELISA: Boll Antibody (3E10) [H00066037-M03]

ELISA: Boll Antibody (3E10) [H00066037-M03] - Detection limit for recombinant GST tagged BOLL is approximately 0.3ng/ml as a capture antibody.

Applications for Boll Antibody (3E10)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Boll

This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Alternate Names

bol, boule-like (Drosophila), BOULEbol (Drosophila boule homolog)-like, protein boule-like

Entrez Gene IDs

66037 (Human)

Gene Symbol

BOLL

OMIM

606165 (Human)

UniProt

NP_149019 (Human)

Additional Boll Products

Product Documents for Boll Antibody (3E10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Boll Antibody (3E10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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