Boll Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33538

Novus Biologicals, part of Bio-Techne
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NBP2-33538
NBP2-33538-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Boll Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: VTFETQEDAQKILQEAEKLNYKDKKLNIGPAIRKQQVGIPRSSIMPAAGTMYLTTSTGYPYTYHNGVAYFHTPEVTSVPPPWPSRSVCSSPVMVAQPIYQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Boll Antibody

Western Blot: Boll Antibody [NBP2-33538]

Western Blot: Boll Antibody [NBP2-33538]

Western Blot: Boll Antibody [NBP2-33538] - Analysis in control (vector only transfected HEK293T lysate) and BOLL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538] - Staining of human lymph node.
Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
View 6 more images

Applications for Boll Antibody

Application
Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Boll

Boll belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Alternate Names

bol, boule-like (Drosophila), BOULEbol (Drosophila boule homolog)-like, protein boule-like

Gene Symbol

BOLL

UniProt

Q8N9W6 (Human)

Additional Boll Products

Product Documents for Boll Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Boll Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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