BoNT-A Light Chain Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12385
Key Product Details
Species Reactivity
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Antibody Source
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Product Specifications
Immunogen
Clonality
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Scientific Data Images for BoNT-A Light Chain Antibody
Western Blot: BoNT-A Light Chain Antibody [NBP3-12385]
Western Blot: BoNT-A Light Chain Antibody [NBP3-12385] - A) Rat lung, B) Rat spleen. 1:500 antibody dilution in Dilobffer. Apparent MW is 100 KDa.Immunohistochemistry-Paraffin: BoNT-A Light Chain Antibody [NBP3-12385]
Immunohistochemistry-Paraffin: BoNT-A Light Chain Antibody [NBP3-12385] - Rat small intestine. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.Applications for BoNT-A Light Chain Antibody
ELISA
Immunohistochemistry-Paraffin
Immunoprecipitation
Western Blot
Formulation, Preparation, and Storage
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Background: BoNT-A Light Chain
There are seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum. BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain. BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction.
E. coli-expressed recombinant BoNT light chains are active proteases. However, they are not toxic because they cannot enter into host cells in the absence of the heavy chains.
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Additional BoNT-A Light Chain Products
Product Documents for BoNT-A Light Chain Antibody
Product Specific Notices for BoNT-A Light Chain Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.