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BoNT-A Light Chain Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12385

Novus Biologicals, part of Bio-Techne
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NBP3-12385

Key Product Details

Species Reactivity

Human, Mouse, Rat, Primate

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Synthetic peptide taken within amino acid region 778-818 on human Ectonucleotide pyrophosphatase/phosphodiesterase 2 (autotaxin) protein.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for BoNT-A Light Chain Antibody

Western Blot: BoNT-A Light Chain Antibody [NBP3-12385]

Western Blot: BoNT-A Light Chain Antibody [NBP3-12385]

Western Blot: BoNT-A Light Chain Antibody [NBP3-12385] - A) Rat lung, B) Rat spleen. 1:500 antibody dilution in Dilobffer. Apparent MW is 100 KDa.
Immunohistochemistry-Paraffin: BoNT-A Light Chain Antibody [NBP3-12385]

Immunohistochemistry-Paraffin: BoNT-A Light Chain Antibody [NBP3-12385]

Immunohistochemistry-Paraffin: BoNT-A Light Chain Antibody [NBP3-12385] - Rat small intestine. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.

Applications for BoNT-A Light Chain Antibody

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry-Paraffin

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:250

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: BoNT-A Light Chain

There are seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum. BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain. BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction.

E. coli-expressed recombinant BoNT light chains are active proteases. However, they are not toxic because they cannot enter into host cells in the absence of the heavy chains.

Long Name

Botulinum Neurotoxin Type A Light Chain

Alternate Names

BoNTA Light Chain

Gene Symbol

atx

Additional BoNT-A Light Chain Products

Product Documents for BoNT-A Light Chain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BoNT-A Light Chain Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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