BPOZ Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-836

Novus Biologicals, part of Bio-Techne
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NB100-836
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all BPOZ Antibodies »

Product Specifications

Immunogen

Peptide with sequence RALEDLLVSIGLDC corresponding to C-Terminus according to NP_115937.1, NP_742024.1, NP_742025.1.

Specificity

This antibody is expected to recognise all reported isoforms (NP_115937.1; NP_742024.1; NP_742025.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for BPOZ Antibody

Western Blot: BPOZ Antibody [NB100-836]

Western Blot: BPOZ Antibody [NB100-836]

Western Blot: BPOZ Antibody [NB100-836] - Staining of Human Heart lysate with antibody at 1 ug/mL (RIPA buffer, 35 ug total protein). Primary incubated for 1 hour. Detected by chemiluminescence. Approx 38 kDa band observed in Human Heart lysates, and approx. 50 kDa in preliminary testing of MCF7 cell lysate (calculated MW of 37.6 kDa according to NP_115937.1 and 54 kDa according to ; NP_742024.1). An additional band was also consistently observed at 65-70 kDa. and was successfully blocked by incubation with the immunizing peptide.
Immunohistochemistry-Paraffin: BPOZ Antibody [NB100-836]

Immunohistochemistry-Paraffin: BPOZ Antibody [NB100-836]

Immunohistochemistry-Paraffin: BPOZ Antibody [NB100-836] - Staining of paraffin embedded Human Cerebellum with antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: BPOZ Antibody [NB100-836]

Immunohistochemistry-Paraffin: BPOZ Antibody [NB100-836]

Immunohistochemistry-Paraffin: BPOZ Antibody [NB100-836] - Staining of paraffin embedded Human Heart with antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for BPOZ Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 5 ug/mL

Immunohistochemistry-Paraffin

3 - 5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/mL

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BPOZ

This protein has an ankyrin repeat region and two BTB/POZ domains, which are thought to be involved in protein-protein interactions. Expression of the gene is activated by the phosphatase and tensin homolog, a tumor suppressor. Alternate splicing results in three transcript variants encoding different isoforms.

Alternate Names

ankyrin repeat and BTB (POZ) domain containing 1, ankyrin repeat and BTB/POZ domain-containing protein 1, BPOZMGC20585, Btb3, EF1ABP, elongation factor 1A binding protein, Elongation factor 1A-binding protein

Entrez Gene IDs

80325 (Human); 80283 (Mouse); 498013 (Rat)

Gene Symbol

ABTB1

UniProt

NP_115937. (Human)

Additional BPOZ Products

Product Documents for BPOZ Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BPOZ Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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