Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all Brachyury Antibodies »

Product Summary for Brachyury Antibody

Immunogen

Recognizes region between residues Between between 350 and 400..

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Brachyury Antibody

Brachyury Antibody

Immunoprecipitation;Brachyury Antibody[NBP3-18506]

Immunoprecipitation;Brachyury Antibody Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from NCI-H460 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBXT antibody used for IP at 6 µg per reaction. TBXT was also immunoprecipitated by rabbit anti-TBXT antibody. For bting immunoprecipitated TBXT, was used at 0.4 mg/ml0. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Brachyury Antibody

Application
Recommended Usage

Immunoprecipitation

15-20 ug/mg lysate

Western Blot

1:2000 - 1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Brachyury

Brachyury / Bry is encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

Alternate Names

brachyury protein, MGC104817, Protein T, SAVA, T brachyury (mouse) homolog, T brachyury homolog, T Brachyury Transcription Factor, T, brachyury homolog (mouse), T-Box Transcription Factor T, TBXT, TF, TFT

Gene Symbol

TBXT

Additional Brachyury Products

Product Documents for Brachyury Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Brachyury Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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