Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Brachyury Antibodies »

Product Specifications

Immunogen

Recognizes region between residues Between between 350 and 400..

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Brachyury Antibody - BSA Free

Brachyury Antibody

Immunoprecipitation;Brachyury Antibody[NBP3-18506]

Immunoprecipitation;Brachyury Antibody Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from NCI-H460 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBXT antibody used for IP at 6 µg per reaction. TBXT was also immunoprecipitated by rabbit anti-TBXT antibody. For bting immunoprecipitated TBXT, was used at 0.4 mg/ml0. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Brachyury Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

15-20 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Brachyury

Brachyury / Bry is encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

Alternate Names

brachyury protein, MGC104817, Protein T, SAVA, T brachyury (mouse) homolog, T brachyury homolog, T Brachyury Transcription Factor, T, brachyury homolog (mouse), T-Box Transcription Factor T, TBXT, TF, TFT

Gene Symbol

TBXT

Additional Brachyury Products

Product Documents for Brachyury Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Brachyury Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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