Bradykinin RB1/BDKRB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-96919

Novus Biologicals, part of Bio-Techne
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NB100-96919
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Bradykinin RB1/BDKRB1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence KVWELYKQCTPK corresponding to internal region according to NP_000701.2.

Epitope

KVWELYKQCTPK

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Bradykinin RB1/BDKRB1 Antibody

Western Blot: Bradykinin RB1/BDKRB1 Antibody [NB100-96919]

Western Blot: Bradykinin RB1/BDKRB1 Antibody [NB100-96919]

Western Blot: Bradykinin RB1/BDKRB1 Antibody [NB100-96919] - Analysis of BDKRB1 using NB100-96919 at 2ug/ml in K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Bradykinin RB1/BDKRB1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:1000

Western Blot

2 - 4 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in lysates of Daudi, K562 and U937 calculated MW of 40.5 kDa band according to NP_000701.2). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bradykinin RB1/BDKRB1

Bradykinin, a 9 aa peptide, is generated in pathophysiologic conditions such as inflammation, trauma, burns, shock, and allergy. Two types of G-protein coupled receptors have been found which bind bradykinin and mediate responses to these pathophysiologic conditions. The protein encoded by this gene is one of these receptors and is synthesized de novo following tissue injury. Receptor binding leads to an increase in the cytosolic calcium ion concentration, ultimately resulting in chronic and acute inflammatory responses. [provided by RefSeq]

Long Name

Bradykinin Receptor B1

Alternate Names

B1BKR, BDKRB1, BK-1 Receptor, BKB1R, BKR1, BRADYB1, BradykininRB1

Entrez Gene IDs

623 (Human)

Gene Symbol

BDKRB1

UniProt

NP_000701.2 (Human)

Additional Bradykinin RB1/BDKRB1 Products

Product Documents for Bradykinin RB1/BDKRB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Bradykinin RB1/BDKRB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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