Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all BRAP Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 560 and 592 of human BRCA1 associated protein using the numbering given in entry NP_006759.3 (GeneID 8315).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRAP Antibody

Western Blot: BRAP Antibody [NBP1-42696]

Western Blot: BRAP Antibody [NBP1-42696]

Western Blot: BRAP Antibody [NBP1-42696] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-BRAP antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. BRAP was also immunoprecipitated by rabbit anti-BRAP antibody NBP1-42695, which recognizes an upstream epitope.

Applications for BRAP Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BRAP

The protein encoded by the BRAP gene was identified by its ability to bind to the nuclear localization signal of BRCA1 and other proteins. It is a cytoplasmic protein which may regulate nuclear targeting by retaining proteins with a nuclear localization signal in the cytoplasm. (provided by RefSeq)

Alternate Names

BRAP2RING finger protein 52, BRCA1 associated protein, EC 6.3.2.-, IMPBRCA1-associated protein, Impedes mitogenic signal propagation, Renal carcinoma antigen NY-REN-63, RNF52galectin-2-binding protein

Entrez Gene IDs

8315 (Human)

Gene Symbol

BRAP

Additional BRAP Products

Product Documents for BRAP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BRAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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