BRCA1 Antibody (KEN) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-599

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # KEN

Format

BSA Free

Concentration

1.5 mg/ml

View all BRCA1 Antibodies »

Product Specifications

Immunogen

Human BRCA1 corresponding to residues 1314-1864 [UniProt# P38398]

Epitope

The epitope recognized is found between amino acids 1314-1600.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Theoretical MW

240 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BRCA1 Antibody (KEN) - BSA Free

Western Blot: BRCA1 Antibody (KEN)BSA Free [NB100-599]

Western Blot: BRCA1 Antibody (KEN)BSA Free [NB100-599]

Western Blot: BRCA1 Antibody (KEN) [NB100-599] - Detection of BRCA1 in U2OS whole cell lysate using NB 100-599. 1 minute ECL detection.
Immunocytochemistry/ Immunofluorescence: BRCA1 Antibody (KEN) - BSA Free [NB100-599]

Immunocytochemistry/ Immunofluorescence: BRCA1 Antibody (KEN) - BSA Free [NB100-599]

Immunocytochemistry/Immunofluorescence: BRCA1 Antibody (KEN) [NB100-599] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-BRCA1 (KEN) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: BRCA1 Antibody (KEN)BSA Free [NB100-599]

Western Blot: BRCA1 Antibody (KEN)BSA Free [NB100-599]

Western Blot: BRCA1 Antibody (KEN) [NB100-599] - Detection of BRCA1.

Applications for BRCA1 Antibody (KEN) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunoprecipitation

1:10-1:500

Western Blot

2-4 ug/ml
Application Notes
This BRCA1 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In Western blot a band was observed ~220-240 kDa.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BRCA1

BRCA1 (breast cancer early-onset 1) is a tumour-suppressor gene which was originally discovered on the basis of its linkage to early-onset breast or ovarian cancer in women. BRCA1 gene encodes a protein of 1863 amino acids with three distinct regions of protein interaction: RING domain, RAD51-interaction domain and BRCT (BRCA1 C-terminus) domain. The N-terminal RING finger domain binds to several proteins, including formation of heterodimers with BARD1 (BRCA1-associated RING domain protein). Both BRCA1 and BARD1 contain the RING finger domain which is important for tumour-suppression functions. The RAD51-interaction domain is enclosed by exon 11 and is involved in DNA-DSBs repair. It also contains multiple protein-binding sites, in addition to those for RAD51/RAD50 complex. Loss of RAD51 binding increase the risk of cancer as there could be an increment in the amount of damaged DNA. The C-terminus of BRCA1 contains an acidic domain that functions as a transcriptional activation domain and this domain contains a tandem repeat of approximately 95 amino acids called BRCT. This domain is also found in proteins such as BARD1 which is involved in cell-cycle control and DNA repair. BRCA1 has been implicated in diverse cancer-related activities, including roles in cell-cycle progression, DNA repair, DNA damage-responsive cell-cycle checkpoints, transcription regulation, ubiquitination, chromosome remodelling and apoptosis. Defects in BRCA1 are a cause of susceptibility to breast cancer, familial breast-ovarian cancer type 1 (BROVCA1), ovarian cancer, and pancreatic cancer type 4 (PNCA4).

Long Name

Breast Cancer 1

Alternate Names

BRCAI, breast and ovarian cancer susceptibility protein 1, breast and ovarian cancer sususceptibility protein, breast cancer 1, early onset, breast cancer type 1 susceptibility protein, EC 6.3.2, EC 6.3.2.-, IRIS, PNCA4, PSCP, subunit 1

Gene Symbol

BRCA1

Additional BRCA1 Products

Product Documents for BRCA1 Antibody (KEN) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BRCA1 Antibody (KEN) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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