Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Chimpanzee (100%), Monkey (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all BRCA1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1800 and the C-terminus (residue 1863) of human Breast Cancer Gene 1 using the numbering given in entry NP_009225.1 (GeneID 672).

Reactivity Notes

Gorilla (100%).

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRCA1 Antibody

Western Blot: BRCA1 Antibody [NB100-199]

Western Blot: BRCA1 Antibody [NB100-199]

Western Blot: BRCA1 Antibody [NB100-199] - Whole cell lysate (50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRCA1 antibody used for WB at 0.04ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds
Western Blot: BRCA1 Antibody [NB100-199]

Western Blot: BRCA1 Antibody [NB100-199]

Western Blot: BRCA1 Antibody [NB100-199] - Detection of Human BRCA1 by Western Blot and Immunoprecipitation. Sample: Nuclear extract from HeLa cells. Affinity purified rabbit anti-BRCA1 NB100-199 used at 1 mcg/ml for WB and 4 mcg for IP.

Applications for BRCA1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:1000-1:10000
Application Notes
*The investigator should determine the optimal dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BRCA1

BRCA 1 contains at least two nuclear localization signals and is proposed to be a tumor suppressor protein. It is a serine phosphoprotein that undergoes hyperphosphorylation during late G1 and S phases of the cell cycle and is transiently dephosphorylated early after M phase. BRCA 1 protein alters in a qualitative and quantitative manner during cell cycle progression. The amount of BRCA protein is highest during S phase and remains elevated toward G2 / M, before it declines in early G1 phase. Inherited loss of BRCA 1 function confers an increased susceptibility for both breast and ovarian cancer.

Long Name

Breast Cancer 1

Alternate Names

BRCAI, breast and ovarian cancer susceptibility protein 1, breast and ovarian cancer sususceptibility protein, breast cancer 1, early onset, breast cancer type 1 susceptibility protein, EC 6.3.2, EC 6.3.2.-, IRIS, PNCA4, PSCP, subunit 1

Gene Symbol

BRCA1

Additional BRCA1 Products

Product Documents for BRCA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BRCA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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