Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Chimpanzee (100%), Monkey (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1800 and the C-terminus (residue 1863) of human Breast Cancer Gene 1 using the numbering given in entry NP_009225.1 (GeneID 672).

Reactivity Notes

Gorilla (100%).

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRCA1 Antibody - BSA Free

Western Blot: BRCA1 Antibody [NB100-199]

Western Blot: BRCA1 Antibody [NB100-199]

Western Blot: BRCA1 Antibody [NB100-199] - Whole cell lysate (50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRCA1 antibody used for WB at 0.04ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds
Western Blot: BRCA1 Antibody [NB100-199]

Western Blot: BRCA1 Antibody [NB100-199]

Western Blot: BRCA1 Antibody [NB100-199] - Detection of Human BRCA1 by Western Blot and Immunoprecipitation. Sample: Nuclear extract from HeLa cells. Affinity purified rabbit anti-BRCA1 NB100-199 used at 1 mcg/ml for WB and 4 mcg for IP.

Applications for BRCA1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:1000-1:10000
Application Notes
*The investigator should determine the optimal dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BRCA1

BRCA 1 contains at least two nuclear localization signals and is proposed to be a tumor suppressor protein. It is a serine phosphoprotein that undergoes hyperphosphorylation during late G1 and S phases of the cell cycle and is transiently dephosphorylated early after M phase. BRCA 1 protein alters in a qualitative and quantitative manner during cell cycle progression. The amount of BRCA protein is highest during S phase and remains elevated toward G2 / M, before it declines in early G1 phase. Inherited loss of BRCA 1 function confers an increased susceptibility for both breast and ovarian cancer.

Long Name

Breast Cancer 1

Alternate Names

BRCAI, breast and ovarian cancer susceptibility protein 1, breast and ovarian cancer sususceptibility protein, breast cancer 1, early onset, breast cancer type 1 susceptibility protein, EC 6.3.2, EC 6.3.2.-, IRIS, PNCA4, PSCP, subunit 1

Gene Symbol

BRCA1

Additional BRCA1 Products

Product Documents for BRCA1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BRCA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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