BRCA2 Antibody (5F6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-41189
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5F6
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Amino acids 2336-2478 of human BRCA2 expressed in E. coli. BRCA2-P400.
Localization
Nuclear
Specificity
This antibody will recognize human BRCA2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
384 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for BRCA2 Antibody (5F6)
Western Blot: BRCA2 Antibody (5F6) [NBP1-41189] -
Western Blot: BRCA2 Antibody (5F6) [NBP1-41189] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA2 antibody [5F6] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Applications for BRCA2 Antibody (5F6)
Application
Recommended Usage
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Frozen
1:10 - 1:500
Immunohistochemistry-Paraffin
1:10 - 1:500
Western Blot
1-5 ug/ml
Application Notes
Do not boil samples for longer than two minutes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BRCA2
Long Name
Breast Cancer 2
Alternate Names
BRCA1/BRCA2-containing complex, subunit 2, BRCC2, breast and ovarian cancer susceptibility gene, early onset, breast cancer 2 tumor suppressor, breast cancer 2, early onset, breast cancer susceptibility protein BRCA2, breast cancer type 2 susceptibility protein, BROVCA2, FACD, FAD, FAD1, FANCB, FANCD, FANCD1Fanconi anemia, complementation group D1, Fanconi anemia group D1 protein, GLM3, PNCA2
Entrez Gene IDs
675 (Human)
Gene Symbol
BRCA2
UniProt
Additional BRCA2 Products
Product Documents for BRCA2 Antibody (5F6)
Product Specific Notices for BRCA2 Antibody (5F6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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