Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B1

Format

BSA Free

Concentration

1 mg/ml

View all BRCC3 Antibodies »

Product Specifications

Immunogen

Recombinant BRCC3 (1-316aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for BRCC3 Antibody (3B1) - BSA Free

Western Blot: BRCC3 Antibody (3B1)BSA Free [NBP1-30429]

Western Blot: BRCC3 Antibody (3B1)BSA Free [NBP1-30429]

Western Blot: BRCC3 Antibody (3B1) [NBP1-30429] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat ant imouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: 293T cell lysate Lane 4.: MCF7 cell lysate Lane 5.: NIH3T3 cell lysate.
Immunocytochemistry/ Immunofluorescence: BRCC3 Antibody (3B1) - BSA Free [NBP1-30429]

Immunocytochemistry/ Immunofluorescence: BRCC3 Antibody (3B1) - BSA Free [NBP1-30429]

Immunocytochemistry/Immunofluorescence: BRCC3 Antibody (3B1) [NBP1-30429] - CC/IF analysis of BRCC36 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BRCC36 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for BRCC3 Antibody (3B1) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BRCC3

BRCC36 (BRCA1/BRCA2-containing complex, subunit 36) is a holoenzyme complex containing BRCA1, BRCA2. BRCC36 is an ubiquitin E3 ligase complex that has a role in DNA repair and enhances cellular survival following DNA damage. This protein is also thought to be involved in the cellular response to ionizing radiation and progression through the G2/M checkpoint.

Alternate Names

BRCA1/BRCA2-containing complex subunit 3, BRCA1/BRCA2-containing complex subunit 36, BRCA1/BRCA2-containing complex, subunit 3, BRCA1-A complex subunit BRCC36, BRCC36C6.1ACXorf53, BRISC complex subunit BRCC36, c6.1A, chromosome X open reading frame 53, EC 3.1.2.15, EC 3.4.19.-, lys-63-specific deubiquitinase BRCC36

Entrez Gene IDs

79184 (Human)

Gene Symbol

BRCC3

UniProt

NP_077308 (Human)

Additional BRCC3 Products

Product Documents for BRCC3 Antibody (3B1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BRCC3 Antibody (3B1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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