BRD3 Antibody (6E7)

Novus Biologicals, part of Bio-Techne | Catalog # H00008019-M01

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 6E7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BRD3 Antibodies »

Product Specifications

Immunogen

BRD3 (AAH32124, 418 a.a. ~ 556 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EPVEAPALPAPAAPMVSKGAESSRSSEESSSDSGSSDSEEERATRLAELQEQLKAVHEQLAALSQAPVNKPKKKKEKKEKEKKKKDKEKEKEKHKVKAEEEKKAKVAPPAKQAQQKKAPAKKANSTTTAGRDHFLTCGV

Localization

Nuclear

Specificity

BRD3 - bromodomain containing 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for BRD3 Antibody (6E7)

Western Blot: BRD3 Antibody (6E7) [H00008019-M01]

Western Blot: BRD3 Antibody (6E7) [H00008019-M01] - BRD3 monoclonal antibody (M01), clone 6E7 Analysis of BRD3 expression in Hela S3 NE.

Immunohistochemistry-Paraffin: BRD3 Antibody (6E7) [H00008019-M01]

Immunohistochemistry-Paraffin: BRD3 Antibody (6E7) [H00008019-M01] - Analysis of monoclonal antibody to BRD3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.

Sandwich ELISA: BRD3 Antibody (6E7) [H00008019-M01] - Detection limit for recombinant GST tagged BRD3 is approximately 0.3ng/ml as a capture antibody.

Applications for BRD3 Antibody (6E7)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BRD3

The bromodomain is a conserved 110 amino acid motif which specifically interacts with acetyl lysines, at least in the context of short histone H3 and H4 peptides. Although bromodomains have now been found in more than 40 different proteins, the function of this motif is poorly understood. The association of two N terminal bromodomains with a C terminal extraterminal (ET) domain defines the Fsh/Brd subgroup, which includes members in many species. In vertebrates, four members of the Fsh/Brd subgroup have now been identified: Brd2/RING3/fsrg1, Brd3/ORFX/fsrg2, Brd4/HUNK1/MCAP, and Brd5/BRDT. The Brd3/ORFX gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known.

Long Name

Bromodomain Containing 3

Alternate Names

ORFX, RING3L

Entrez Gene IDs

8019 (Human)

Gene Symbol

BRD3

OMIM

601541 (Human)

UniProt

AAH32124 (Human)

Additional BRD3 Products

Product Documents for BRD3 Antibody (6E7)

Product Datasheets

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COA

SDS

Product Specific Notices for BRD3 Antibody (6E7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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