BRD4 Antibody (BL-149-2H5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76393

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-76393
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Chromatin Immunoprecipitation Sequencing, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BL-149-2H5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all BRD4 Antibodies »

Product Specifications

Immunogen

Immunogen sequence between amino acids 1312 and 1362

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRD4 Antibody (BL-149-2H5)

Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393]

Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393]

Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of mouse BRD4 by WB of BW5147.3 lysate. Antibody: BRD4 Antibody (BL-149-2H5). Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunocytochemistry/ Immunofluorescence: BRD4 Antibody (BL-149-2H5) [NBP2-76393]

Immunocytochemistry/ Immunofluorescence: BRD4 Antibody (BL-149-2H5) [NBP2-76393]

Immunocytochemistry/Immunofluorescence: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] (NBP2-76393). Secondary: DyLight 550-conjugated goat anti-rabbit IgG. Counterstain: Phalloidin Alexa Fluor 488-conjugated (green).
Immunohistochemistry-Paraffin: BRD4 Antibody (BL-149-2H5) [NBP2-76393]

Immunohistochemistry-Paraffin: BRD4 Antibody (BL-149-2H5) [NBP2-76393]

Immunohistochemistry-Paraffin: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Section of breast carcinoma. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
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Applications for BRD4 Antibody (BL-149-2H5)

Application
Recommended Usage

Flow Cytometry

Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl

Immunocytochemistry/ Immunofluorescence

1:25 - 1:250

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

20 ul/mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: BRD4

Bromodomain-containing protein 4 (BRD4) contains 2 bromo-domains. Bromo-domains are found in chromatin-associating proteins and can interact specifically with acetylated lysine. BRD4 is described as a chromatin adaptor and has been shown to bind acetylated chromatin. It was originally identified as MCAP (mitotic chromosome associated protein) due to its association with mitotic chromosomes. BRD4 also associates with several transcription complexes such as the Mediator complexes and P-TEFb complexes and is considered to function as a transcriptional co-factor.

Long Name

Bromodomain Containing 4

Alternate Names

HUNK1, HUNKI, MCAP

Gene Symbol

BRD4

Additional BRD4 Products

Product Documents for BRD4 Antibody (BL-149-2H5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BRD4 Antibody (BL-149-2H5)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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