BRD4 Antibody (BL-151-6F11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76394

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-76394
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BL-151-6F11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all BRD4 Antibodies »

Product Summary for BRD4 Antibody (BL-151-6F11)

Immunogen

Immunogen sequence between amino acids 1312 and 1362

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRD4 Antibody (BL-151-6F11)

Western Blot: BRD4 Antibody (BL-151-6F11) [NBP2-76394]

Western Blot: BRD4 Antibody (BL-151-6F11) [NBP2-76394]

Western Blot: BRD4 Antibody (BL-151-6F11) [NBP2-76394] - Whole cell lysate (50 ug) from BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] usedat 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry/ Immunofluorescence: BRD4 Antibody (BL-151-6F11) [NBP2-76394]

Immunocytochemistry/ Immunofluorescence: BRD4 Antibody (BL-151-6F11) [NBP2-76394]

Immunocytochemistry/Immunofluorescence: BRD4 Antibody (BL-151-6F11) [NBP2-76394] - Detection of human BRD4 (red) in formaldelyde-fixed Hep-G2 cells by ICC-IF. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-151-6F11] (NBP2-76394). Secondary: DyLight 594-conjugated goat anti-rabbit IgG. Counterstain: Phalloidin Alexa Fluor 488-conjugated (green) and DAPI (blue).
Immunohistochemistry-Paraffin: BRD4 Antibody (BL-151-6F11) [NBP2-76394]

Immunohistochemistry-Paraffin: BRD4 Antibody (BL-151-6F11) [NBP2-76394]

Immunohistochemistry-Paraffin: BRD4 Antibody (BL-151-6F11) [NBP2-76394] - Rabbit anti-BRD4 recombinant monoclonal [BL-151-6F11]. (Secondary: HRPconjugated goat anti-rabbit IgG Substrate: DAB.
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Applications for BRD4 Antibody (BL-151-6F11)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:25 - 1:250

Immunohistochemistry

1:25 - 1:250

Immunoprecipitation

20 ul/mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ICC/IF Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. FLOW Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: BRD4

Bromodomain-containing protein 4 (BRD4) contains 2 bromo-domains. Bromo-domains are found in chromatin-associating proteins and can interact specifically with acetylated lysine. BRD4 is described as a chromatin adaptor and has been shown to bind acetylated chromatin. It was originally identified as MCAP (mitotic chromosome associated protein) due to its association with mitotic chromosomes. BRD4 also associates with several transcription complexes such as the Mediator complexes and P-TEFb complexes and is considered to function as a transcriptional co-factor.

Long Name

Bromodomain Containing 4

Alternate Names

HUNK1, HUNKI, MCAP

Gene Symbol

BRD4

Additional BRD4 Products

Product Documents for BRD4 Antibody (BL-151-6F11)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BRD4 Antibody (BL-151-6F11)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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