BRD4 Antibody (CL1115)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52958

Novus Biologicals, part of Bio-Techne
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NBP2-52958
NBP2-52958-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1115

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BRD4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PQPAKPQQVIQHHHSPRHHKSDPYSTGHLREAPSPLMIHSPQMSQFQSLTHQSPPQQNVQPKKQELRAASVVQPQPLVVVKEEKIHSPIIRSEPFSPSLRPEPPKHPESIKAPVHLPQRPEMKPVDVGRPVIRPPEQNAPPP

Epitope

PQPAKPQQVIQHHHS

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for BRD4 Antibody (CL1115)

Western Blot: BRD4 Antibody (CL1115) [NBP2-52958]

Western Blot: BRD4 Antibody (CL1115) [NBP2-52958]

Western Blot: BRD4 Antibody (CL1115) [NBP2-52958] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-BRD4 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Western Blot: BRD4 Antibody (CL1115) [NBP2-52958]

Western Blot: BRD4 Antibody (CL1115) [NBP2-52958]

Western Blot: BRD4 Antibody (CL1115) [NBP2-52958] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: BRD4 Antibody (CL1115) [NBP2-52958]

Immunocytochemistry/ Immunofluorescence: BRD4 Antibody (CL1115) [NBP2-52958]

Immunocytochemistry/Immunofluorescence: BRD4 Antibody (CL1115) [NBP2-52958] - Staining of MCF-7 cells using the Anti-BRD4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
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Applications for BRD4 Antibody (CL1115)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BRD4

BRD4 is encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq]

Long Name

Bromodomain Containing 4

Alternate Names

HUNK1, HUNKI, MCAP

Gene Symbol

BRD4

Additional BRD4 Products

Product Documents for BRD4 Antibody (CL1115)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BRD4 Antibody (CL1115)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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