BRD4 Antibody (RAB-C131) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20125
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # RAB-C131
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was obtained by recombinant antibody (rAb) phage display recognizing BRD4 protein under non-denaturing conditions.
Epitope
This antibody binds to a folded domain, amino acids 333-460.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for BRD4 Antibody (RAB-C131) - Chimeric - Azide and BSA Free
Flow Cytometry: BRD4 Antibody (RAB-C131) - Chimeric - Azide and BSA Free [NBP3-20125] -
Flow Cytometry: BRD4 Antibody (RAB-C131) - Chimeric - Azide and BSA Free [NBP3-20125] - HepG2 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-C131 (NBP3-20125, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunocytochemistry/Immunofluorescence: BRD4 Antibody (RAB-C131) - Chimeric - Azide and BSA Free [NBP3-20125] -
Immunocytochemistry/Immunofluorescence: BRD4 Antibody (RAB-C131) - Chimeric - Azide and BSA Free [NBP3-20125] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RAB-C131 (NBP3-20125) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom NBP3-20125, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.
Western Blot: BRD4 Antibody (RAB-C131) - Chimeric - Azide and BSA Free [NBP3-20125] -
Western Blot: BRD4 Antibody (RAB-C131) - Chimeric - Azide and BSA Free [NBP3-20125] - Nuclear lysate of K562 cells (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-C131 (NBP3-20125) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.Applications for BRD4 Antibody (RAB-C131) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: BRD4
Long Name
Bromodomain Containing 4
Alternate Names
HUNK1, HUNKI, MCAP
Gene Symbol
BRD4
Additional BRD4 Products
Product Documents for BRD4 Antibody (RAB-C131) - Chimeric - Azide and BSA Free
Product Specific Notices for BRD4 Antibody (RAB-C131) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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