Breast carcinoma amplified sequence 3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-40682

Novus Biologicals, part of Bio-Techne
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NB110-40682
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all Breast carcinoma amplified sequence 3 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 870 and the C-terminus (residue 928) of human Breast Carcinoma Amplified Sequence 3 using the numbering given in entry BAB15156.1 (GeneID 54828).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Breast carcinoma amplified sequence 3 Antibody

Western Blot: Breast carcinoma amplified sequence 3 Antibody [NB110-40682]

Western Blot: Breast carcinoma amplified sequence 3 Antibody [NB110-40682]

Western Blot: Breast carcinoma amplified sequence 3 Antibody [NB110-40682] - Detection of Human BCAS3 on HeLa whole cell lysates using NB110-40682. BCAS3 was also immunoprecipitated by another rabbit anti-BCAS3 antibody.
Immunohistochemistry: Breast carcinoma amplified sequence 3 Antibody [NB110-40682]

Immunohistochemistry: Breast carcinoma amplified sequence 3 Antibody [NB110-40682]

Immunohistochemistry: Breast carcinoma amplified sequence 3 Antibody [NB110-40682] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BCAS3 used at a dilution of 1:200 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry-Paraffin: Breast carcinoma amplified sequence 3 Antibody [NB110-40682]

Immunohistochemistry-Paraffin: Breast carcinoma amplified sequence 3 Antibody [NB110-40682]

Immunohistochemistry-Paraffin: Breast carcinoma amplified sequence 3 Antibody [NB110-40682] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-BCAS3 used at a dilution of 1:500. DAB staining using IHC Accessory Kit
View 1 more images

Applications for Breast carcinoma amplified sequence 3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:2000-1:10000
Application Notes
This antibody is useful for Western Blot Immunohistochemistry-Paraffin and Immunoprecipitation. Epitope exposure is recommended. Epitope exposure with citrate pH6.0 buffer or tris-EDTA pH9.0 buffer will enhance staining. Likely to work with frozen sections

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Breast carcinoma amplified sequence 3

BCAS3 acts as an ERalpha coactivator in breast cancer cells. It has been demonstrated that PELP1, a newly described ERalpha coregulator, is recruited to BCAS3 chromatin and activates its expression. Analysis of the BCAS3 sequence for functional motifs and evidence from biochemical fractionation suggested that BCAS3 acts as a transcriptional coactivator. Results from chromatin immunoprecipitation, reporter assays, and expression studies further validated the coactivator function of BCAS3 for ERalpha. BCAS3 physically associated with histone H3 and histone acetyltransferase complex protein P/CAF and possessed histone acetyltransferase activity.

Alternate Names

BCAS4/BCAS3 fusion, breast carcinoma amplified sequence 3, breast carcinoma amplified sequence 4/3 fusion protein, breast carcinoma-amplified sequence 3, DKFZp686O1527, FLJ20128, GAOB1, MAAB, metastasis associated antigen of breast cancer, MGC4973, protein Maab1

Entrez Gene IDs

54828 (Human)

Gene Symbol

BCAS3

UniProt

BAB15156.1 (Human)

Additional Breast carcinoma amplified sequence 3 Products

Product Documents for Breast carcinoma amplified sequence 3 Antibody

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Certificate of Analysis

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Product Specific Notices for Breast carcinoma amplified sequence 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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