Breast carcinoma amplified sequence 3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98807

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Breast carcinoma amplified sequence 3 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Breast carcinoma amplified sequence 3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Breast carcinoma amplified sequence 3 Antibody

Western Blot: Breast carcinoma amplified sequence 3 Antibody [NBP2-98807]

Western Blot: Breast carcinoma amplified sequence 3 Antibody [NBP2-98807]

Western Blot: Breast carcinoma amplified sequence 3 Antibody [NBP2-98807] - Anti-Breast carcinoma amplified sequence 3 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 101 kDa. Observed band size: 113 kDa (We are unsure as to the identity of these extra bands).
Immunocytochemistry/ Immunofluorescence: Breast carcinoma amplified sequence 3 Antibody [NBP2-98807]

Immunocytochemistry/ Immunofluorescence: Breast carcinoma amplified sequence 3 Antibody [NBP2-98807]

Immunocytochemistry/Immunofluorescence: Breast carcinoma amplified sequence 3 Antibody [NBP2-98807] - Immunofluorescence staining of Breast carcinoma amplified sequence 3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human Breast carcinoma amplified sequence 3 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.

Applications for Breast carcinoma amplified sequence 3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Breast carcinoma amplified sequence 3

BCAS3 acts as an ERalpha coactivator in breast cancer cells. It has been demonstrated that PELP1, a newly described ERalpha coregulator, is recruited to BCAS3 chromatin and activates its expression. Analysis of the BCAS3 sequence for functional motifs and evidence from biochemical fractionation suggested that BCAS3 acts as a transcriptional coactivator. Results from chromatin immunoprecipitation, reporter assays, and expression studies further validated the coactivator function of BCAS3 for ERalpha. BCAS3 physically associated with histone H3 and histone acetyltransferase complex protein P/CAF and possessed histone acetyltransferase activity.

Alternate Names

BCAS4/BCAS3 fusion, breast carcinoma amplified sequence 3, breast carcinoma amplified sequence 4/3 fusion protein, breast carcinoma-amplified sequence 3, DKFZp686O1527, FLJ20128, GAOB1, MAAB, metastasis associated antigen of breast cancer, MGC4973, protein Maab1

Gene Symbol

BCAS3

Additional Breast carcinoma amplified sequence 3 Products

Product Documents for Breast carcinoma amplified sequence 3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Breast carcinoma amplified sequence 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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