Brg1 Antibody (BRG1/6531R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20647
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # BRG1/6531R
Concentration
0.2 mg/ml
Product Summary for Brg1 Antibody (BRG1/6531R)
Immunogen
Recombinant fragment (around aa 200-400) of human Brg1 protein (exact sequence is proprietary)
Localization
Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: HeLa or HEK-293T cells. Ubiquitous nuclear expression.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for Brg1 Antibody (BRG1/6531R)
Brg1 Antibody (BRG1/6531R)
Formalin-fixed, paraffin-embedded human colon stained with Brg1 antibody (BRG1/6531R). Inset: PBS instead of primary antibody; secondary only negative control.
Brg1 Antibody (BRG1/6531R)
Formalin-fixed, paraffin-embedded human ovarian small cell carcinoma stained with Brg1 antibody (BRG1/6531R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Brg1 Antibody (BRG1/6531R)
Formalin-fixed, paraffin-embedded human kidney stained with Brg1 antibody (BRG1/6531R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for Brg1 Antibody (BRG1/6531R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Brg1
Long Name
BRM/SWI2-related gene 1
Alternate Names
SMARCA4, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2
Gene Symbol
SMARCA4
Additional Brg1 Products
Product Documents for Brg1 Antibody (BRG1/6531R)
Product Specific Notices for Brg1 Antibody (BRG1/6531R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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