Brg1 Antibody (CL13779)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24575

Novus Biologicals, part of Bio-Techne
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NBP3-24575-100ul
NBP3-24575-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL13779

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Brg1 Antibodies »

Product Specifications

Immunogen

This antibody was designed to target the recombinant protein Brg1, with the precise immunogen sequence being withheld for proprietary reasons.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Brg1 Antibody (CL13779)

Immunocytochemistry/Immunofluorescence: Brg1 Antibody (CL13779) [NBP3-24575]

Staining in HeLa cell line with Anti-SMARCA4 monoclonal antibody, showing specific staining of nucleoplasm and nuclear bodies in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Western Blot: Brg1 Antibody (CL13779) [NBP3-24575]

Western blot analysis in human cell line MCF-7.

Applications for Brg1 Antibody (CL13779)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Western Blot

1ug/ml
Application Notes
For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Brg1

BRG1 is encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.

Long Name

BRM/SWI2-related gene 1

Alternate Names

SMARCA4, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2

Gene Symbol

SMARCA4

Additional Brg1 Products

Product Documents for Brg1 Antibody (CL13779)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Brg1 Antibody (CL13779)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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