Brg1 Antibody (CL13783)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24576

Novus Biologicals, part of Bio-Techne
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NBP3-24576-100ul
NBP3-24576-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL13783

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Brg1 Antibodies »

Product Specifications

Immunogen

This antibody was designed to target the recombinant protein Brg1, with the precise immunogen sequence being withheld for proprietary reasons.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Brg1 Antibody (CL13783)

Immunohistochemistry-Paraffin: Brg1 Antibody (CL13783) [NBP3-24576]

Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: Brg1 Antibody (CL13783) [NBP3-24576]

Analysis in human testis and liver tissues using NBP3-24576 antibody. Corresponding SMARCA4 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Brg1 Antibody (CL13783) [NBP3-24576]

Staining of human endometrial cancer shows strong nuclear positivity in tumor cells.
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Applications for Brg1 Antibody (CL13783)

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Brg1

BRG1 is encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.

Long Name

BRM/SWI2-related gene 1

Alternate Names

SMARCA4, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2

Gene Symbol

SMARCA4

Additional Brg1 Products

Product Documents for Brg1 Antibody (CL13783)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Brg1 Antibody (CL13783)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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