BRIP1/FANCJ Antibody

Immunocytochemistry/Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - 

Immunocytochemistry/Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - Expression of BRIP1/FANCJ at the mRNA (graphs) and protein (photos) levels in iPSCs. Gene expression was assessed with RNA-seq in FACS-sorted bone marrow HSCs or with real-time RT-PCR in cultured iPSCs. The box-and-whiskers graphs show the median, 25th and 75th percentile (box) minimum, and maximum values (whiskers) of the FPKM (fragments per kilobase million) or relative expression in comparison to house-keeping genes (geometric mean for HPRT, B2M, and beta-actin), n = 4, * p < 0.05, ** p < 0.01, Mann–Whitney test (HSCs) or Wilcoxon test (iPSCs). Nuclei are labeled with DAPI. Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33445471/) licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] -

Immunocytochemistry/ Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - In situ proximity ligation assay (PLA) in HEK293T cells. (A) G4-BRIP1 & (B) G4-HMOX1 interactions (red dots) were visualized in the control group & after DNase or RNase digestion. Most of the G4 interactions were localized in the nucleus (arrows), but some were also present in the cytoplasm. Cells that were fixed in methanol & mouse 1H6 antibodies were used. Nuclei were counterstained with DAPI (blue), where the transmission bright-field images (BF) show the cell morphologies. Scale bar: 10 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33445471), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] -

Immunocytochemistry/ Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - Detection of the PLA signal in sorted hematopoietic stem cells. (A) G4–Brip1 & (B) G4–Hmox1 interactions (green dots) were visualized in HSCs & GMP & MPP cells. G4 interactions were visible in the nucleus (arrows) & in the cytoplasm. Nuclei were counterstained with DAPI (blue), where the transmission bright-field images (BF) show the cell morphologies. Cells were fixed in PFA & goat 1H6 antibodies were used. Scale bars: 5 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33445471), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] -

Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRIP1/FANCJ antibody [N1N2], N-term (NBP1-31883) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.