Brk Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89936

Novus Biologicals, part of Bio-Techne
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NBP1-89936
NBP1-89936-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Brk Antibodies »

Product Summary for Brk Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PYPGMSNHEAFLRVDAGYRMPCPLECPPSVHKLMLTCWCRDPEQRPCFKALRERLSSFTSYENPT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Brk Antibody

Western Blot: Brk Antibody [NBP1-89936]

Western Blot: Brk Antibody [NBP1-89936]

Western Blot: Brk Antibody [NBP1-89936] - Analysis in control (vector only transfected HEK293T lysate) and PTK6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry: Brk Antibody [NBP1-89936]

Immunohistochemistry: Brk Antibody [NBP1-89936]

Immunohistochemistry: Brk Antibody [NBP1-89936] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells and decidua cells.
Western Blot: Brk Antibody [NBP1-89936]

Western Blot: Brk Antibody [NBP1-89936]

Western Blot: Brk Antibody [NBP1-89936] - Analysis in human cell line TD47D.

Applications for Brk Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:100 - 1:250
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Brk

Brk is encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. [provided by RefSeq]

Long Name

Breast Tumor Kinase

Alternate Names

PTK6, Sik, Tksk

Gene Symbol

PTK6

Additional Brk Products

Product Documents for Brk Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Brk Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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