BRN3A Antibody (7B4)

Novus Biologicals, part of Bio-Techne | Catalog # H00005457-M04

Novus Biologicals, part of Bio-Techne
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H00005457-M04
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 7B4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BRN3A Antibodies »

Product Specifications

Immunogen

POU4F1 (NP_006228.2, 331 a.a. ~ 420 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QAWLEEAEGAQREKMNKPELFNGGEKKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKFSATY

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for BRN3A Antibody (7B4)

Western Blot: BRN3A Antibody (7B4) [H00005457-M04]

Western Blot: BRN3A Antibody (7B4) [H00005457-M04]

Western Blot: BRN3A Antibody (7B4) [H00005457-M04] - Analysis of POU4F1 expression in transfected 293T cell line by POU4F1 monoclonal antibody (M04), clone 7B4. Lane 1: POU4F1 transfected lysatE (46.09 KDa). Lane 2: Non-transfected lysate.
ELISA: BRN3A Antibody (7B4) [H00005457-M04]

ELISA: BRN3A Antibody (7B4) [H00005457-M04]

ELISA: BRN3A Antibody (7B4) [H00005457-M04] - Detection limit for recombinant GST tagged POU4F1 is 3 ng/ml as a capture antibody.

Applications for BRN3A Antibody (7B4)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA and WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BRN3A

Brn-3a is a transcription factor belonging to the class IV of POU domain transcription factors. It is expressed throughout the peripheral nervous system but especially in postmitotic sensory neurons of dorsal root ganglia. Brn-3a is known to regulate different genes involved in neuronal differentiation and survival (1). Characterization of Brn-3a suggests potential roles of Brn-3a in the development of retinal ganglion cells and sensory neurons, as well as potential roles in the pituitary gland and the immune system. Brn-3a is expressed in the pituitary gland and in a corticotroph cell line (2). The differentiation of the ND7 neuronal cell line to a nondividing phenotype bearing numerous neurite processes is accompanied by a dramatic increase in the levels of the activating POU family transcription factor Brn-3a and a corresponding fall in the levels of the closely related inhibitory factor Brn-3b. Over expression of Brn-3a in these cells can induce neurite outgrowth and the activation of genes encoding synaptic vesicle proteins in the absence of a differentiation-inducing stimulus (3).

Alternate Names

Brain-3A, Brain-specific homeobox/POU domain protein 3A, brn-3A, BRN3Abrain-3A, FLJ13449, Homeobox/POU domain protein RDC-1, Oct-T1, POU class 4 homeobox 1, POU domain class 4, transcription factor 1, POU domain, class 4, transcription factor 1, RDC1, RDC-1

Gene Symbol

POU4F1

UniProt

NP_006228 (Human)

Additional BRN3A Products

Product Documents for BRN3A Antibody (7B4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BRN3A Antibody (7B4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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