Bromodeoxyuridine/BrdU Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB500-235
![Immunocytochemistry/ Immunofluorescence: Bromodeoxyuridine/BrdU Antibody [NB500-235]](https://resources.bio-techne.com/images/products/Bromodeoxyuridine-BrdU-Antibody-Immunocytochemistry-Immunofluorescence-NB500-235-img0006.jpg "Immunocytochemistry/ Immunofluorescence: Bromodeoxyuridine/BrdU Antibody [NB500-235]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, All Species, Guinea Pig
Cited:
Human, Mouse, Rat, Guinea Pig, Rabbit
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro assay
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vitro assay
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Concentration
3.2 mg/ml
Product Specifications
Immunogen
Bromodeoxyuridine (BrdU) coupled to keyhole limpet hemocyanin (KLH).
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:33731750).
Specificity
BrdU
Marker
Proliferation Marker
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Bromodeoxyuridine/BrdU Antibody
![Immunoprecipitation: Bromodeoxyuridine/BrdU Antibody [NB500-235]](https://resources.bio-techne.com/images/products/Bromodeoxyuridine-BrdU-Antibody-Immunoprecipitation-NB500-235-img0005.jpg "Immunoprecipitation: Bromodeoxyuridine/BrdU Antibody [NB500-235]")
Immunoprecipitation: Bromodeoxyuridine/BrdU Antibody [NB500-235]
Immunoprecipitation: Bromodeoxyuridine/BrdU Antibody [NB500-235] - Lysate was immunoprecipitated with anti-brdu antibody (NB500-235), 5-methyl cytosine (5-Me), or a no antibody control (No A). An ELISA was then performed to measure total activity.Applications for Bromodeoxyuridine/BrdU Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:200
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
10 ug/ml
Immunohistochemistry-Paraffin
10 ug/ml
Immunoprecipitation
25-100 ug/ml
Application Notes
Use in IHC-Fr reported in scientific literature (PMID:33731750). Use in IHC-FrFl reported in scientific literature (PMID:33744708) In vitro reported in (PMID: 29100301).
Reviewed Applications
Read 2 reviews rated 5 using NB500-235 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4)
Preservative
No Preservative
Concentration
3.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Bromodeoxyuridine/BrdU
Alternate Names
5-bromo-2-deoxyuridine, BrdU
Additional Bromodeoxyuridine/BrdU Products
Product Documents for Bromodeoxyuridine/BrdU Antibody
Product Specific Notices for Bromodeoxyuridine/BrdU Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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