Brorin/VWC2 Antibody (03) [Janelia Fluor® 635]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05796JF635

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA Detection

Label

Janelia Fluor 635

Antibody Source

Monoclonal Mouse IgG Clone # 03

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Brorin/VWC2 Antibodies »

Product Specifications

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Brorin/VWC2 (Accession#: NP_940972.2; Met1-Met325).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Applications for Brorin/VWC2 Antibody (03) [Janelia Fluor® 635]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA Detection

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Brorin/VWC2

Brorin (brain-specific chordin-like protein), also called VWC2, is an ~46 kDa glycoprotein that is a member of the Chordin family of secreted BMP regulators. The human Brorin cDNA encodes 325 amino acids (aa) including a 27 aa signal sequence and a 298 aa secreted mature protein with two VWFC domains. These domains contain a pattern of 10 cysteine residues that is conserved in other family members, with the remaining aa sequence sharing little identity. Human Brorin shares 90%, 91%, and 95% aa sequence identity with mouse, rat, and equine Brorin, respectively. It also shares aa identity with VWC2L (Brorin-like) of 37% overall and 62% within the VWFC domains. Brorin is predominantly expressed in embryonic and adult neural tissues in the mouse. Expression of Brorin mRNA is concentrated in neurons within the diencephalon and medulla oblongata but is not detected in the developing cerebral cortex. Brorin binds and antagonizes BMPs, interacting via the VWFC domains. It promotes neurogenesis in mouse neural precursors. Knockdown of Brorin in zebrafish embryos results in morphological abnormalities in the brain and eye.

Long Name

von Willebrand Factor A2 Domain

Alternate Names

Brain-specific chordin-like protein, VWC2

Gene Symbol

VWC2

Additional Brorin/VWC2 Products

Product Documents for Brorin/VWC2 Antibody (03) [Janelia Fluor® 635]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Brorin/VWC2 Antibody (03) [Janelia Fluor® 635]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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