Brorin/VWC2 Antibody (08)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05797

Novus Biologicals, part of Bio-Techne
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NBP3-05797-100ul
NBP3-05797-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 08

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Brorin/VWC2 Antibodies »

Product Specifications

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Brorin/VWC2 (Accession#: NP_940972.2; Met1-Met325).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Applications for Brorin/VWC2 Antibody (08)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA Capture

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody will detect Human Brorin/VWC2 in ELISA pair set (Catalog: # NBP2-79385). In a sandwich ELISA, it can be used as a capture antibody when paired with (Catalog: # NBP3-05796).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Brorin/VWC2

Brorin (brain-specific chordin-like protein), also called VWC2, is an ~46 kDa glycoprotein that is a member of the Chordin family of secreted BMP regulators. The human Brorin cDNA encodes 325 amino acids (aa) including a 27 aa signal sequence and a 298 aa secreted mature protein with two VWFC domains. These domains contain a pattern of 10 cysteine residues that is conserved in other family members, with the remaining aa sequence sharing little identity. Human Brorin shares 90%, 91%, and 95% aa sequence identity with mouse, rat, and equine Brorin, respectively. It also shares aa identity with VWC2L (Brorin-like) of 37% overall and 62% within the VWFC domains. Brorin is predominantly expressed in embryonic and adult neural tissues in the mouse. Expression of Brorin mRNA is concentrated in neurons within the diencephalon and medulla oblongata but is not detected in the developing cerebral cortex. Brorin binds and antagonizes BMPs, interacting via the VWFC domains. It promotes neurogenesis in mouse neural precursors. Knockdown of Brorin in zebrafish embryos results in morphological abnormalities in the brain and eye.

Long Name

von Willebrand Factor A2 Domain

Alternate Names

Brain-specific chordin-like protein, VWC2

Gene Symbol

VWC2

Additional Brorin/VWC2 Products

Product Documents for Brorin/VWC2 Antibody (08)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Brorin/VWC2 Antibody (08)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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