BRP44L Antibody

Immunohistochemistry-Paraffin: BRP44L Antibody [NBP1-91706]

Immunohistochemistry-Paraffin: BRP44L Antibody [NBP1-91706] - Staining of human kidney shows moderate to strong positivity in mitochondria in cells in tubules.

Western Blot: BRP44L Antibody [NBP1-91706]

Western Blot: BRP44L Antibody [NBP1-91706] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue

Immunohistochemistry-Paraffin: BRP44L Antibody [NBP1-91706]

Immunohistochemistry-Paraffin: BRP44L Antibody [NBP1-91706] - Staining of human duodenum shows strong positivity in mitochondria in glandular cells.

Immunohistochemistry-Paraffin: BRP44L Antibody [NBP1-91706]

Immunohistochemistry-Paraffin: BRP44L Antibody [NBP1-91706] - Staining of human heart muscle shows strong positivity in mitochondria in cardiomyocytes.

Immunohistochemistry-Paraffin: BRP44L Antibody [NBP1-91706]

Immunohistochemistry-Paraffin: BRP44L Antibody [NBP1-91706] - Staining of human pancreas shows weak positivity in exocrine glandular cells as expected.

Immunohistochemistry-Paraffin: BRP44L Antibody [NBP1-91706]

Immunohistochemistry-Paraffin: BRP44L Antibody [NBP1-91706] - Staining of human salivary gland shows strong positivity in mitochondria in ductal cells.

Knockout Validated: BRP44L Antibody [NBP1-91706]

Knockout Validated: BRP44L Antibody [NBP1-91706] - Characterization of MPC1 (BRP44L) -/- in the RM-1 murine prostate cancer cells. Western blotting results of subcellular proteins from WT and MPC1 (BRP44L) -/- cells and corresponding densitometry histograms of ALT1 and PC are shown. Cyto means cytoplasmic protein. Mito stands for mitochondrial protein. Protein alpha-Tubulin was used as a loading control. ***P<0.001. All experiments were performed at least three times with consistent and repeatable results. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/fulltext/18199), licensed under a CC-BY license.

Western Blot: BRP44L Antibody [NBP1-91706] -

Western Blot: BRP44L Antibody [NBP1-91706] - The results of western blotting & RT-PCR assayA. shows Western blotting results, where the PDHA1 protein expression in the parental LNCaP cells is strongly positive, but its expression in the PDHA1 KO cells is almost negative with two different PDHA1 antibodies. Comparatively, the protein expression of the MPC1 & MPC2 is comparatively decreased & the protein expression of the GLUT1, HK2, LDHA, HIF1 alpha, GLS1 & GLUD1 is increased in the LNCaP PDHA1 KO cells. GAPDH was added as a loading control. B. shows the RT-PCR results of the mRNA expression of GLUD1 & GLS1, where the expression of the GLUD1 & GLS1 is significantly increased in the PDHA1 KO cells, with P values of 0.000 & 0.001, respectively, compared to the parental LNCaP cells. **P < 0.01, ***P<0.001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27462778), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BRP44L Antibody [NBP1-91706] -

Western Blot: BRP44L Antibody [NBP1-91706] - Characterization of MPC1−/− in the RM-1 murine prostate cancer cells(A) Shows representative sequencings of the MPC1 PCR products in WT & MPC1−/− cells. The upper panel shows a representative MPC1 PCR product sequencing chart of WT cells at the left part, & the right part on the upper panel shows the full cDNA sequence & the speculated amino acids (110 amino acids). The left part on the lower panel shows a representative MPC1 PCR product of the MPC1−/− cells, which reveals double sets of peak. The right part of the lower panel shows the mutated DNA sequences at both alleles, on which one allele on the upper part has a two-base “at” deletion (green arrow), while another allele on the lower part harbors one base “a” insertion (red arrow). Both mutated sequences create an early terminator “tga”, which results in only 1/3 of the translation (41 amino acids in the upper allele & 42 amino cods in another allele). (B) The IF & IHC results. Co-localization of MPC1 or MPC2 & the mitochondrial protein MT-CO1 are shown in the WT RM-1 cells, while there is no MPC1 protein expression revealed by either IF or ICC in the MPC1−/− cells. There was only weak MPC2 protein expression in the MPC1−/− cells revealed by ICC. Scale bars, 200 μm. Western blotting results of subcellular proteins from WT & MPC1−/− cells & corresponding densitometry histograms of ALT1 & PC are shown in (C). Cyto means cytoplasmic protein. Mito stands for mitochondrial protein. Protein alpha-Tublin was used as a loading control. ***P<0.001. All experiments were performed at least three times with consistent & repeatable results. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28624784), licensed under a CC-BY license. Not internally tested by Novus Biologicals.