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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In vivo assay

Cited:

IF/IHC, Immunohistochemistry Free-Floating, Immunohistochemistry-Paraffin, In vivo assay, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 kappa Clone # 120G8.04

Concentration

0.5 mg/ml

Product Specifications

Immunogen

mouse plasmatocytoid DCs (pDCs)

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 31339007).

Specificity

Mouse pDCs/BST-2/IFN producing cells (IPC) (extracellular domain)

Clonality

Monoclonal

Host

Rat

Isotype

IgG1 kappa

Scientific Data Images for BST2 Antibody (120G8.04)

Immunocytochemistry/ Immunofluorescence: BST2 Antibody (120G8.04) [DDX0390P-100]

Immunocytochemistry/ Immunofluorescence: BST2 Antibody (120G8.04) [DDX0390P-100]

Immunocytochemistry/Immunofluorescence: BST2 Antibody (120G8.04) [DDX0390P-100] - Murine peyer patch: BST-2
Immunohistochemistry: BST2 Antibody (120G8.04) [DDX0390P-100]

Immunohistochemistry: BST2 Antibody (120G8.04) [DDX0390P-100]

Immunohistochemistry: BST2 Antibody (120G8.04) [DDX0390P-100] - IHC staining of murine spleen cryosections
Flow Cytometry: BST2 Antibody (120G8.04) [DDX0390P-100]

Flow Cytometry: BST2 Antibody (120G8.04) [DDX0390P-100]

Flow Cytometry: BST2 Antibody (120G8.04) [DDX0390P-100] - In vivo depletion of mouse PDCs (gated on CD11c+CD3- cells).

Applications for BST2 Antibody (120G8.04)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10 - 1:500
Application Notes
Monoclonal antibody 120G8.04 may be used between at 1-10 ug/ml. For pDCs in vivo depletion in Balb /c mice, mAb 120G8 was used between 50-200 ug / injection. Use in Immunohistochemistry free floating reported in scientific literature (PMID: 31207335).

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

Tris-NaCl (pH 8.0)

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BST-2/Tetherin

Bone marrow stromal cells are involved in the growth and development of B-cells. The specific function of the protein encoded by the bone marrow stromal cell antigen 2 is undetermined; however, this protein may play a role in pre-B-cell growth and in rheumatoid arthritis. [provided by RefSeq]

Long Name

Bone Marrow Stromal Cell Antigen 2

Alternate Names

BST2, CD317, HM1.24 Antigen, NPC-A-7, Tetherin

Gene Symbol

BST2

Additional BST-2/Tetherin Products

Product Documents for BST2 Antibody (120G8.04)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BST2 Antibody (120G8.04)

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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