BST2 Antibody (4F6) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29622
![Immunohistochemistry-Paraffin: BST2 Antibody (4F6) - BSA Free [NBP2-29622]](https://resources.bio-techne.com/images/products/BST2-Antibody-4F6-Immunohistochemistry-Paraffin-NBP2-29622-img0002.jpg "Immunohistochemistry-Paraffin: BST2 Antibody (4F6) - BSA Free [NBP2-29622]")
![Western Blot: BST2 Antibody (4F6)BSA Free [NBP2-29622]](https://resources.bio-techne.com/images/products/BST2-Antibody-4F6-Western-Blot-NBP2-29622-img0001.jpg "Western Blot: BST2 Antibody (4F6)BSA Free [NBP2-29622]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4F6
Format
BSA Free
Concentration
1.0 mg/ml
Product Summary for BST2 Antibody (4F6) - BSA Free
Immunogen
Full length recombinant human BST2 protein [NCBI NP_004326].
Localization
Cell membrane, trans-Golgi network, late endosome and cytoplasm
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for BST2 Antibody (4F6) - BSA Free
Western Blot: BST2 Antibody (4F6)BSA Free [NBP2-29622]
Western Blot: BST2 Antibody (4F6) [NBP2-29622] - WB analysis of BST2 protein in (A) human heart lysate (B) human ovary lysate and on (C) partial recombinant BST2 protein with monoclonal BST2 antibody (clone 4F6) at a concentration of 1 ug/mL. In the tested lysates, two bands were observed which represents the glycosylated (lower intense bands) and the ubiquitinated (higher weak bands) forms of BST2 protein.![Immunohistochemistry-Paraffin: BST2 Antibody (4F6) - BSA Free [NBP2-29622]](https://resources.bio-techne.com/images/products/BST2-Antibody-4F6-Immunohistochemistry-Paraffin-NBP2-29622-img0003.jpg "Immunohistochemistry-Paraffin: BST2 Antibody (4F6) - BSA Free [NBP2-29622]")
Immunohistochemistry-Paraffin: BST2 Antibody (4F6) - BSA Free [NBP2-29622]
Immunohistochemistry-Paraffin: BST2 Antibody (4F6) [NBP2-29622] - Analysis of BST2 protein in a section of human lung cancer (squamous cell carcinoma) using 5 ug/mL concentration of BST2 antibody (clone 4F6). The carcinoma cells depicted strong membrane-cytoplasmic BST2 positivity with more intense staining in the cellular membranes, and a relatively less intense staining pattern was observed in the tumor stroma.![Flow (Cell Surface): BST2 Antibody (4F6) - BSA Free [NBP2-29622]](https://resources.bio-techne.com/images/products/BST2-Antibody-4F6-Flow-Cell-Surface-NBP2-29622-img0004.jpg "Flow (Cell Surface): BST2 Antibody (4F6) - BSA Free [NBP2-29622]")
Flow (Cell Surface): BST2 Antibody (4F6) - BSA Free [NBP2-29622]
Flow (Cell Surface): BST2 Antibody (4F6) [NBP2-29622] - Flow analysis of Molt4 cells using the BST2 antibody [4F6]. The shaded purple region represents cells alone, green represents the isotype control and pink represents the BST2 antibody.Applications for BST2 Antibody (4F6) - BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 ug/mL
Immunohistochemistry
5 ug/mL
Immunohistochemistry-Paraffin
5 ug/mL
Western Blot
1.0 ug/mL
Application Notes
The unprocessed form of BST2 protein is 180 amino acids long (161 amino acids in the cleaved BST) and its cleaved form undergoes further modifications namely disulfide bond formation, GPI-anchor/GPI-like-anchor binding, glycosylation, isopeptide bond formation, lipidation and Ubl conjugation. The predicted molecular weight of unmodified BST2 protein is ~17-18 kDa, whereas the post-translationally modified protein has been documented to run at ~25-36 kDa (glycosylated), 50-55 kDa (ubiquitinated) and 65-70 kDa (dimers). BST2 protein localizes mainly to the cell membranes from where it shuttles to the trans-Golgi network, late endosome and the cytoplasm. In immuno-staining assays, BST2 may develop a membrane-cytoplasmic staining pattern. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BST-2/Tetherin
Long Name
Bone Marrow Stromal Cell Antigen 2
Alternate Names
BST2, CD317, HM1.24 Antigen, NPC-A-7, Tetherin
Entrez Gene IDs
684 (Human)
Gene Symbol
BST2
Additional BST-2/Tetherin Products
Product Documents for BST2 Antibody (4F6) - BSA Free
Product Specific Notices for BST2 Antibody (4F6) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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