BTAF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57502

Novus Biologicals, part of Bio-Techne
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NB100-57502
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all BTAF1 Antibodies »

Product Summary for BTAF1 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1050 and 1100 of human RNA polymerase II, B-TFIID transcription factor-associated, 170kDa using the numbering given in entry NP_003963.1 (GeneID 9044).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BTAF1 Antibody

Immunoprecipitation: BTAF1 Antibody [NB100-57502]

Immunoprecipitation: BTAF1 Antibody [NB100-57502]

Immunoprecipitation: BTAF1 Antibody [NB100-57502] - Detection of Human BTAF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20 percent of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTAF1 antibody NB100-57502 used for IP at 3 mcg/mg lysate (A and B). BTAF1 was also immunoprecipitated by rabbit anti-BTAF1 antibodies NB100-57501 and NB100-57503, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 30 seconds (B).

Applications for BTAF1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BTAF1

BTAF1 is a member of the SNF2-like family of ATPases that associates with the TATA-binding protein (TBP) and TAFII170 (TAF172) to form the B-TF-IID complex. As part of the general transcription machinery, BTAF1 acts as a regulator of TBP and RNA polymerase II transcription. Alternate names for BTAF1 include BTAF-1, RNA polymerase II, B-TFIID transcription factor-associated, 170kDa, TAF172, TBP-associated factor 172, TAFII170, B-TFIID transcription factor-associated, 170kDa, and MOT1.

Alternate Names

ATP-dependent helicase BTAF1, BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1homolog, S. cerevisiae), B-TFIID transcription factor-associated 170 kDa subunit, EC 3.6.1, EC 3.6.4.-, MOT1MGC138406, TAF(II)170KIAA0940, TAF-172, TAF172BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170 kD (Mot1homolog, S. cerevisiae), TAFII170TATA-binding protein-associated factor 172, TBP-associated factor 172

Entrez Gene IDs

9044 (Human)

Gene Symbol

BTAF1

UniProt

NP_003963.1 (Human)

Additional BTAF1 Products

Product Documents for BTAF1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BTAF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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