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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 35 and 70 of basic transcription factor 3 using the numbering given in entry NP_001032726.1 (GeneID 689).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BTF3 Antibody

Immunohistochemistry: BTF3 Antibody [NBP1-28700]

Immunohistochemistry: BTF3 Antibody [NBP1-28700]

Immunohistochemistry: BTF3 Antibody [NBP1-28700] - Sample: FFPE section of human breast carcinoma (left) and human prostate carcinoma (right). Antibody: Affinity purified rabbit anti- BTF3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: BTF3 Antibody [NBP1-28700]

Immunoprecipitation: BTF3 Antibody [NBP1-28700]

Immunoprecipitation: BTF3 Antibody [NBP1-28700] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTF3 antibody NBP1-28700 used for IP at 10 ug/mg lysate. BTF3 was also immunoprecipitated by rabbit anti-BTF3 antibody NBP1-28716, which recognizes an upstream epitope. For blotting immunoprecipitated BTF3, NBP1-28716 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for BTF3 Antibody

Application
Recommended Usage

Immunoprecipitation

10 ug/mg lysate
Application Notes
It is not recommended for western analysis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BTF3

Basic transcription factor 3 (BTF3) is a general transcription factor that forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation.

Long Name

Basic Transcription Factor 3

Alternate Names

BETA-NAC, NACB

Entrez Gene IDs

689 (Human)

Gene Symbol

BTF3

Additional BTF3 Products

Product Documents for BTF3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BTF3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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