BTF3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49225

Novus Biologicals, part of Bio-Techne
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NBP2-49225
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BTF3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: TSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BTF3 Antibody

Western Blot: BTF3 Antibody [NBP2-49225]

Western Blot: BTF3 Antibody [NBP2-49225]

Western Blot: BTF3 Antibody [NBP2-49225] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: BTF3 Antibody [NBP2-49225]

Immunocytochemistry/ Immunofluorescence: BTF3 Antibody [NBP2-49225]

Immunocytochemistry/Immunofluorescence: BTF3 Antibody [NBP2-49225] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: BTF3 Antibody [NBP2-49225]

Immunohistochemistry-Paraffin: BTF3 Antibody [NBP2-49225]

Immunohistochemistry-Paraffin: BTF3 Antibody [NBP2-49225] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules.

Applications for BTF3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BTF3

Basic transcription factor 3 (BTF3) is a general transcription factor that forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation.

Long Name

Basic Transcription Factor 3

Alternate Names

BETA-NAC, NACB

Gene Symbol

BTF3

Additional BTF3 Products

Product Documents for BTF3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BTF3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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