Bub1 Antibody (1A11C7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61719

Novus Biologicals, part of Bio-Techne
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NBP2-61719
NBP2-61719-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1A11C7

Format

BSA Free

Concentration

1 mg/ml

View all Bub1 Antibodies »

Product Summary for Bub1 Antibody (1A11C7) - BSA Free

Immunogen

Purified recombinant fragment of human BUB1 (AA: 1-130) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

122 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Bub1 Antibody (1A11C7) - BSA Free

Western Blot: Bub1 Antibody (1A11C7)BSA Free [NBP2-61719]

Western Blot: Bub1 Antibody (1A11C7)BSA Free [NBP2-61719]

Western Blot: Bub1 Antibody (1A11C7) [NBP2-61719] - Analysis using BUB1 mAb against human BUB1 (AA: 1-130) recombinant protein. (Expected MW is 41.3 kDa)
Flow Cytometry: Bub1 Antibody (1A11C7) - BSA Free [NBP2-61719]

Flow Cytometry: Bub1 Antibody (1A11C7) - BSA Free [NBP2-61719]

Flow Cytometry: Bub1 Antibody (1A11C7) [NBP2-61719] - Analysis of Hela cells using BUB1 mouse mAb (green) and negative control (red).
Western Blot: Bub1 Antibody (1A11C7)BSA Free [NBP2-61719]

Western Blot: Bub1 Antibody (1A11C7)BSA Free [NBP2-61719]

Western Blot: Bub1 Antibody (1A11C7) [NBP2-61719] - Analysis using BUB1 mAb against HEK293 (1) and BUB1 (AA: 1-130)-hIgGFc transfected HEK293 (2) cell lysate.
View 1 more images

Applications for Bub1 Antibody (1A11C7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bub1

This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.;

Long Name

BUB1 Budding Uninhibited By Benzimidazoles 1 Homolog

Alternate Names

BUB1 budding uninhibited by benzimidazoles 1 homolog, BUB1Amitotic checkpoint serine/threonine-protein kinase BUB1, BUB1L, budding uninhibited by benzimidazoles 1 (yeast homolog), budding uninhibited by benzimidazoles 1 homolog (yeast), EC 2.7.11.1, hBUB1mitotic spindle checkpoint kinase, putative serine/threonine-protein kinase

Gene Symbol

BUB1

Additional Bub1 Products

Product Documents for Bub1 Antibody (1A11C7) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Bub1 Antibody (1A11C7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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