Bub1 Antibody (2F9)
Novus Biologicals, part of Bio-Techne | Catalog # H00000699-M02
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2F9
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
BUB1 (AAH28201, 1 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDTPENVLQMLEAHMQSYKGNDPLGEWERYIQWVEENFPENKEYLITLLEHLMKEFLDKKKYHNDPRFISYCLKFAEYNSDLHQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRGIQNQA
Specificity
BUB1 - BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Bub1 Antibody (2F9)
![Western Blot: Bub1 Antibody (2F9) [H00000699-M02]](https://resources.bio-techne.com/images/products/Bub1-Antibody-2F9-Western-Blot-H00000699-M02-img0007.jpg "Western Blot: Bub1 Antibody (2F9) [H00000699-M02]")
Western Blot: Bub1 Antibody (2F9) [H00000699-M02]
Western Blot: Bub1 Antibody (2F9) [H00000699-M02] - Analysis of BUB1 expression in transfected 293T cell line by BUB1 monoclonal antibody (M02), clone 2F9.Lane 1: BUB1 transfected lysate(122 KDa).Lane 2: Non-transfected lysate.![Western Blot: Bub1 Antibody (2F9) [H00000699-M02]](https://resources.bio-techne.com/images/products/Bub1-Antibody-2F9-Western-Blot-H00000699-M02-img0006.jpg "Western Blot: Bub1 Antibody (2F9) [H00000699-M02]")
Western Blot: Bub1 Antibody (2F9) [H00000699-M02]
Western Blot: Bub1 Antibody (2F9) [H00000699-M02] - BUB1 monoclonal antibody (M02), clone 2F9 Analysis of BUB1 expression in Hela S3 NE.![Immunoprecipitation: Bub1 Antibody (2F9) [H00000699-M02]](https://resources.bio-techne.com/images/products/Bub1-Antibody-2F9-Immunoprecipitation-H00000699-M02-img0004.jpg "Immunoprecipitation: Bub1 Antibody (2F9) [H00000699-M02]")
Immunoprecipitation: Bub1 Antibody (2F9) [H00000699-M02]
Immunoprecipitation: Bub1 Antibody (2F9) [H00000699-M02] - Analysis of BUB1 transfected lysate using anti-BUB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BUB1 MaxPab rabbit polyclonal antibody.Applications for Bub1 Antibody (2F9)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Bub1
Long Name
BUB1 Budding Uninhibited By Benzimidazoles 1 Homolog
Alternate Names
BUB1 budding uninhibited by benzimidazoles 1 homolog, BUB1Amitotic checkpoint serine/threonine-protein kinase BUB1, BUB1L, budding uninhibited by benzimidazoles 1 (yeast homolog), budding uninhibited by benzimidazoles 1 homolog (yeast), EC 2.7.11.1, hBUB1mitotic spindle checkpoint kinase, putative serine/threonine-protein kinase
Entrez Gene IDs
699 (Human)
Gene Symbol
BUB1
OMIM
602452 (Human)
UniProt
Additional Bub1 Products
Product Documents for Bub1 Antibody (2F9)
Product Specific Notices for Bub1 Antibody (2F9)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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