Bub1 Antibody (4C3-4C9)

Novus Biologicals, part of Bio-Techne | Catalog # H00000699-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4C3-4C9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Bub1 Antibodies »

Product Specifications

Immunogen

BUB1 (AAH28201.1, 1 a.a. ~ 1085 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDTPENVLQMLEAHMQSYKGNDPLGVWERYIQWVEENFPENKEYLITLLEHLMKEFLDKKKYHNDPRFISYCLKFAEYNSDLRQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRGIQNQAEPREFLQQQYRLFQTRLTETHLPAQARTSEPLHNVQVLNQMITSKSNPGNNMACISKNQGSELSGVISSACDKESNMERRVITISKSEYSVHSSLASKVDVEQVVMYCKEKLIRGESEFSFEELRAQKYNQRRKHEQWVNEDRHYMKRKEANAFEEQLLKQKMDELHKKLHQVVETSHEDLPASQERSEVNPARMGPSVGSQQELRAPCLPVTYQQTPVNMEKNPREAPPVVPPLANAISAALVSPATSQSIAPPVPLKAQTVTDSMFAVASKDAGCVNKSTHEFKPQSGAEIKEGCETHKVANTSSFHTTPNTSLGMVQATPSKVQPSPTVHTKEALGFIMNMFQAPTLPDISDDKDEWQSLDQNEDAFEAQFQKNVRSSGAWGVNKIISSLSSAFHVFEDGNKENYGLPQPKNKPTGARTFGERSVSRLPSKPKEEVPHAEEFLDDSTVWGIRCNKTLAPSPKSPGDFTSAAQLASTPFHKLPVESVHILEDKENVVAKQCTQATLDSCEENMVVPSRDGKFSPIQEKSPKQALSSHMYSASLLRLSQPAAGGVLTCEAELGVEACRLTDTDAAIAEDPPDAIAGLQAEWMQMSSLGTVDAPNFIVGNPWDDKLIFKLLSGLSKPVSSYPNTFEWQCKLPAIKPKTEFQLGSKLVYVHHLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMERLKPSMQHMFMKFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMRMLYMIEQVHDCEIIHGDIKPDNFILGNGFLEQDDEDDLSAGLALIDLGQSIDMKLFPKGTIFTAKCETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLFGTYMKVKNEGGECKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKVFQQHYTNKIRALRNRLIVLLLECKRSRK

Specificity

BUB1 - BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Bub1 Antibody (4C3-4C9)

Western Blot: Bub1 Antibody (4C3-4C9) [H00000699-M01]

Western Blot: Bub1 Antibody (4C3-4C9) [H00000699-M01]

Western Blot: Bub1 Antibody (4C3-4C9) [H00000699-M01] - Analysis of BUB1 expression in transfected 293T cell line by BUB1 monoclonal antibody (M01), clone 4C3-4C9.Lane 1: BUB1 transfected lysate(122.4 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Bub1 Antibody (4C3-4C9) [H00000699-M01]

Immunocytochemistry/ Immunofluorescence: Bub1 Antibody (4C3-4C9) [H00000699-M01]

Immunocytochemistry/Immunofluorescence: Bub1 Antibody (4C3-4C9) [H00000699-M01] - Analysis of monoclonal antibody to BUB1 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: Bub1 Antibody (4C3-4C9) [H00000699-M01] - Detection limit for recombinant GST tagged BUB1 is approximately 0.03ng/ml as a capture antibody.

Applications for Bub1 Antibody (4C3-4C9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been use for IF.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Bub1

This gene encodes a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer.

Long Name

BUB1 Budding Uninhibited By Benzimidazoles 1 Homolog

Alternate Names

BUB1 budding uninhibited by benzimidazoles 1 homolog, BUB1Amitotic checkpoint serine/threonine-protein kinase BUB1, BUB1L, budding uninhibited by benzimidazoles 1 (yeast homolog), budding uninhibited by benzimidazoles 1 homolog (yeast), EC 2.7.11.1, hBUB1mitotic spindle checkpoint kinase, putative serine/threonine-protein kinase

Entrez Gene IDs

699 (Human)

Gene Symbol

BUB1

OMIM

602452 (Human)

UniProt

AAH28201 (Human)

Additional Bub1 Products

Product Documents for Bub1 Antibody (4C3-4C9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Bub1 Antibody (4C3-4C9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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