Bub1 Antibody (6U8G10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33408
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 6U8G10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human Bub1 (NP_004327.1).
Sequence:
MDTPENVLQMLEAHMQSYKGNDPLGEWERYIQWVEENFPENKEYLITLLEHLMKEFLDKKKYHNDPRFISYCLKFAEYNSDLHQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRGIQNQAEPREFLQQQYRLFQTRLTET
Sequence:
MDTPENVLQMLEAHMQSYKGNDPLGEWERYIQWVEENFPENKEYLITLLEHLMKEFLDKKKYHNDPRFISYCLKFAEYNSDLHQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRGIQNQAEPREFLQQQYRLFQTRLTET
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Bub1 Antibody (6U8G10)
Western Blot: Bub1 Antibody (6U8G10) [NBP3-33408] -
Western Blot: Bub1 Antibody (6U8G10) [NBP3-33408] - Western blot analysis of lysates from HEL cells, using [KD Validated] Bub1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Applications for Bub1 Antibody (6U8G10)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Bub1
Long Name
BUB1 Budding Uninhibited By Benzimidazoles 1 Homolog
Alternate Names
BUB1 budding uninhibited by benzimidazoles 1 homolog, BUB1Amitotic checkpoint serine/threonine-protein kinase BUB1, BUB1L, budding uninhibited by benzimidazoles 1 (yeast homolog), budding uninhibited by benzimidazoles 1 homolog (yeast), EC 2.7.11.1, hBUB1mitotic spindle checkpoint kinase, putative serine/threonine-protein kinase
Gene Symbol
BUB1
Additional Bub1 Products
Product Specific Notices for Bub1 Antibody (6U8G10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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