Bub1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31584

Novus Biologicals, part of Bio-Techne
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NBP1-31584
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Bub1 Antibodies »

Product Summary for Bub1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human BUB1. The exact sequence is proprietary.

Reactivity Notes

Bovine (80%).

Localization

Nucleus, Kinetochore

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

122 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Bub1 Antibody

Western Blot: Bub1 Antibody [NBP1-31584]

Western Blot: Bub1 Antibody [NBP1-31584]

Western Blot: Bub1 Antibody [NBP1-31584] - A. 30 ug 293T whole cell lysate/extract 5 % SDS-PAGE BUB1 antibody dilution: 1:1000
Immunocytochemistry/ Immunofluorescence: Bub1 Antibody [NBP1-31584]

Immunocytochemistry/ Immunofluorescence: Bub1 Antibody [NBP1-31584]

Immunocytochemistry/Immunofluorescence: BUB1 Antibody [NBP1-31584] - Paraformaldehyde-fixed U2OS, using BUB1 antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.
Western Blot: Bub1 Antibody [NBP1-31584]

Western Blot: Bub1 Antibody [NBP1-31584]

Western Blot: BUB1 Antibody [NBP1-31584] - Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE, antibody diluted at 1:3000.
View 1 more images

Applications for Bub1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Bub1

Chromosome segregation and mitotic checkpoints are regulated by various proteins among them are BUB1 and BUBR1/BUB1B (Budding Uninhibited by Benzimidazoles 1). During mitosis both BUB1 and BUBR1 proteins are localized to kinetochores and inhibit the anaphase stage until all chromosomes are correctly attached to the spindle. These proteins were found due to their homology to Bub1 protein from yeast. Human BUBR1 contains CD1 and CD2 domains that are found in human, mouse and yeast BUB1.3-5 The human and mouse BUBR1 proteins are 29% identical in these regions. The kinetochore localization and Bub3 binding is found in the CD1 region while the kinase domain is found in the CD2 domain. While both human and mouse BUB1 contain a nuclear localization site between CD1 and CD2 regions, the yeast protein does not contain this site. BUB1 is expressed in many tissues with the highest expression in testis and thymus, lower expression in colon and small intestine and very low expression in spleen and ovary. BUB1 expression is in correlation with the expression of MAD2, another human spindle assembly checkpoint protein.3-5 BUBR1 is mainly expressed in testis, thymus and bone marrow while in differentiated tissues such as brain, heart and muscle the expression is low.5

Long Name

BUB1 Budding Uninhibited By Benzimidazoles 1 Homolog

Alternate Names

BUB1 budding uninhibited by benzimidazoles 1 homolog, BUB1Amitotic checkpoint serine/threonine-protein kinase BUB1, BUB1L, budding uninhibited by benzimidazoles 1 (yeast homolog), budding uninhibited by benzimidazoles 1 homolog (yeast), EC 2.7.11.1, hBUB1mitotic spindle checkpoint kinase, putative serine/threonine-protein kinase

Gene Symbol

BUB1

Additional Bub1 Products

Product Documents for Bub1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Bub1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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