Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Bub1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Bub1. The immunogen is located within amino acids 40 - 90 of Bub1. Amino Acid Squence: KEFLDKKKYHNDPRFISY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bub1 Antibody - BSA Free

Western Blot: Bub1 AntibodyBSA Free [NBP1-76797]

Western Blot: Bub1 AntibodyBSA Free [NBP1-76797]

Western Blot: Bub1 Antibody [NBP1-76797] - A-20 lysate with bub1 antibody at (A) 2 and (B) 4 ug/ml.
Immunocytochemistry/ Immunofluorescence: Bub1 Antibody - BSA Free [NBP1-76797]

Immunocytochemistry/ Immunofluorescence: Bub1 Antibody - BSA Free [NBP1-76797]

Immunocytochemistry/Immunofluorescence: Bub1 Antibody [NBP1-76797] - A20 cells with Bub1 antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: Bub1 Antibody - BSA Free [NBP1-76797]

Immunocytochemistry/ Immunofluorescence: Bub1 Antibody - BSA Free [NBP1-76797]

Immunocytochemistry/Immunofluorescence: Bub1 Antibody [NBP1-76797] - A20 cells with Bub1 antibody at 10 ug/ml.
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Applications for Bub1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/ml

Western Blot

2-4 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bub1

Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.

Long Name

BUB1 Budding Uninhibited By Benzimidazoles 1 Homolog

Alternate Names

BUB1 budding uninhibited by benzimidazoles 1 homolog, BUB1Amitotic checkpoint serine/threonine-protein kinase BUB1, BUB1L, budding uninhibited by benzimidazoles 1 (yeast homolog), budding uninhibited by benzimidazoles 1 homolog (yeast), EC 2.7.11.1, hBUB1mitotic spindle checkpoint kinase, putative serine/threonine-protein kinase

Gene Symbol

BUB1

UniProt

O43683

Additional Bub1 Products

Product Documents for Bub1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Bub1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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