Bub3 Antibody (10G1S6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16709

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16709-100ul
NBP3-16709-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10G1S6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Bub3 Antibodies »

Product Summary for Bub3 Antibody (10G1S6)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bub3 (O43684). MTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMRLKYQHTGAVLDCAFYDPTHAWSGGLDHQLKMHDLNTDQENLVGTHDAPIR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bub3 Antibody (10G1S6)

Western Blot: Bub3 Antibody (10G1S6) [NBP3-16709]

Western Blot: Bub3 Antibody (10G1S6) [NBP3-16709]

Western Blot: Bub3 Antibody (10G1S6) [NBP3-16709] - Western blot analysis of extracts of various cell lines, using Bub3 Rabbit mAb (NBP3-16709) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: Bub3 Antibody (10G1S6) [NBP3-16709]

Immunocytochemistry/ Immunofluorescence: Bub3 Antibody (10G1S6) [NBP3-16709]

Immunocytochemistry/Immunofluorescence: Bub3 Antibody (10G1S6) [NBP3-16709] - Immunofluorescence analysis of U-2 OS cells using Bub3 Rabbit mAb (NBP3-16709) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Bub3 Antibody (10G1S6) [NBP3-16709]

Immunohistochemistry-Paraffin: Bub3 Antibody (10G1S6) [NBP3-16709]

Immunohistochemistry-Paraffin: Bub3 Antibody (10G1S6) [NBP3-16709] - Immunohistochemistry of paraffin-embedded mouse testis using Bub3 Rabbit mAb (NBP3-16709) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for Bub3 Antibody (10G1S6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bub3

The mitotic checkpoint protein Bub3 appears to be essential during normal mitotic progression, preventing premature sister chromatid separation, missegreation and aneuploidy. Bub3 is also important during G2 and early mitosis stages, promoting normal mitotic entry. Loss of Bub3 function causes slower progression through prophase, apparently due to mitotic cyclins A and B not being accumulated.

Long Name

BUB3 Budding Uninhibited By Benzimidazoles 1 Homolog

Alternate Names

BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog, BUB3 budding uninhibited by benzimidazoles 3 homolog, BUB3L, budding uninhibited by benomyl, budding uninhibited by benzimidazoles 3 homolog (yeast), hBUB3, mitotic checkpoint component, mitotic checkpoint protein BUB3

Gene Symbol

BUB3

Additional Bub3 Products

Product Documents for Bub3 Antibody (10G1S6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Bub3 Antibody (10G1S6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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